Breeding method for intermediate type light/temperature-sensitive genic sterile line of japonica rice

A photo-thermosensitive nuclear sterility, indica-japonica technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of long growth period, low seed setting rate, difficult application, etc., and achieves overcoming the hybrid seed setting rate. low effect

Inactive Publication Date: 2017-12-15
ZHEJIANG NONGKE SEED IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is an effective way to further increase the rice yield level by using indica-japonica heterosis, but typical indica-japonica hybrids often have many unfavorable performances such as too long growth period and low seed setting rate, which make it difficult to apply the hybrid generation in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] (1) Creation of intermediate indica and japonica materials: F1 was obtained by crossing indica rice materials with high quality rice and japonica rice materials, and F1 was backcrossed with indica rice materials once to obtain BC1F1 with 3 / 4 indica genetic components, Plant BC1F1 to obtain BC1F2, plant BC1F2 populations, and select materials from the segregating progeny of indica-japonica intermediate indica-japonica partial indica with compact plant type, plant height of 65 cm, long panicle type, medium-long grain type, darker leaf color, and weak photosensitivity , formed stably after 4 generations of self-crossing reselection;

[0012] (2) Introduction of photothermosensitive genic male sterile gene: use the above selected and stable indica-japonica intermediate type partial indica breeding material to cross with the japonica photothermosensitive genic male sterile line as the female parent to obtain the introduced photothermophilic male sterile line F1 of GMS gene; ...

Embodiment 2

[0015] (1) Creation of intermediate indica and japonica materials: F1 was obtained by crossing indica rice materials with high quality rice and japonica rice materials, and F1 was backcrossed with indica rice materials once to obtain BC1F1 with 3 / 4 indica genetic components, Plant BC1F1 to obtain BC1F2, plant BC1F2 populations, and select materials from the segregated offspring of indica, japonica, partial indica with compact plant type, plant height of 75 cm, long panicle type, medium-long grain type, darker leaf color, and weak photosensitivity , formed stably after 5 generations of self-crossing reselection;

[0016] (2) Introduction of photothermosensitive genic male sterile gene: use the above selected and stable indica-japonica intermediate type partial indica breeding material to cross with the japonica photothermosensitive genic male sterile line as the female parent to obtain the introduced photothermophilic male sterile line F1 of GMS gene;

[0017] (3), breeding of...

Embodiment 3

[0019] Breeding of indica-japonica intermediate photothermo-sensitive GMMS line Zheke 82S: F1 was obtained by crossing CP231, a high-quality indica rice material, with Zaojing 305, a japonica rice material with weak photosensitivity and early maturity, and then CP231 was used as a female This backcross once, obtain BC1F1, harvest and plant BC1F2 population, select from the segregation progeny the indica-japonica intermediate indica-japonica partial indica-type material with good plant leaf shape, long grain shape, dark leaf color and weak photosensitivity, After 4-5 generations of self-crossing reselection, a stable indica-japonica intermediate-indica material GS2066 was obtained. The japonica-type photosensitive GMS line 7001S was used as the female parent, and the indica-japonica intermediate material GS2066 was crossed to introduce the GMS gene and obtain F1. Under the long-day high-temperature conditions of the positive season, the selection in the F2 population conformed t...

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Abstract

The invention belongs to the field of rice breeding, discloses a breeding method for an intermediate type light / temperature-sensitive genic sterile line of japonica rice and relates to the utilization for the advantages of two-line japonica hybrid of rice. The invention aims to utilize a light / temperature-sensitive genic sterile gene to breed the intermediate type light / temperature-sensitive genic sterile line of japonica rice through the japonica hybrid. The two-line sterile line has japonica rice gene components at a certain ratio and can be matched with a hsien rice restoring line for breeding the two-line japonica hybrid of rice with strong heterosis. The proportion of the japonica rice gene components is less, the light sensitivity is weak and the seed production risk is reduced. On the basis of the characteristics of freedom and being not constrained by the restoring and maintaining relationship of the two-line japonica hybrid of rice, the application of the genic sterile line for breeding the two-line japonica hybrid of rice is capable of realizing the utilization for the heterosis of the two-line japonica hybrid of rice and greatly widening the limitation for the advanced genetic resource utilization of the two-line japonica hybrid of rice.

Description

technical field [0001] The invention belongs to the field of rice breeding, in particular to the utilization of heterosis between indica and japonica subspecies of two-line hybrid rice. Background technique [0002] The photothermosensitive genic male sterile lines widely used in my country are mainly the derivative lines of Nongken 58S, Annong S-1 and Hengnong S-1, such as Peiai 64S and Y58S. CMS lines from other sources such as Guangzhan 63S and Zhu 1S are also included. From the phylogenetic relationship of the existing photothermosensitive genic male sterile lines, most of the two-line male sterile lines are related to japonica rice. For example, Pei'ai 64S has the genetic component of japonica Nongken 58, and Y58S is derived from Pei'ai 64S. However, from the perspective of its phenotype, the photothermosensitive genic male sterile lines currently used in large areas are all indica two-line male sterile lines, so that the yield level of the existing two-line hybrid ric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 余守武李梅珍黄益峰曹栋栋
Owner ZHEJIANG NONGKE SEED IND
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