Beijing mountain ash tissue culture method

Abstract

The invention relates to the technical field of plant tissue culture, in particular to a Beijing mountain ash tissue culture method for quickly raising and breeding seedlings. The method comprises the steps that fresh and tender stem segments with buds are obtained from aseptic seedlings and processed and grafted to an initial culture medium for culture, and detoxified stem segments with buds are obtained; multiplication culture is performed, wherein the detoxified stem segments with buds are grafted to a multiplication culture medium containing MS, 1.25 mg/L of 6-BA, 0.25 mg/L of NAA, 0.25mg/L of IBA, 30g/L of sucrose and 6.5-7g/L of agar powder, and culture is performed to obtain sub-cultured seedlings; rooting culture is performed, wherein the sub-cultured seedlings are transferred to a rooting culture medium containing MS, 1.25 mg/L of IBA, 0.05mg/L of NAA, 30g/L of sucrose and 6.5-7g/L of agar, and culture is performed to obtain rooting seedlings; the seedlings are hardened and transplanted, wherein the rooting seedlings are transferred to a greenhouse for seedling hardening, and transplantation is performed after seedling hardening. By adopting the method, the Beijing mountain ash multiplication efficiency can be improved to 26.5, the rooting rate can be up to 93.3%, the survival rate of the hardened seedlings is 100%, and the method is conductive to quantified production of Beijing mountain ash.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of woody plants, in particular to a method for rapid propagation of Beijing Sorbus tissue culture, which belongs to the technical field of tissue culture and seedling cultivation of woody plants. Background technique [0002] Beijing Sorbus (Sorbus discolor) is one of the unique tree species in Rosaceae (Sorbus L.) in China. Its leaf color changes with the seasons, and its fruit is white or yellow. It has great garden ornamental value and medicinal value (Xin Baoying, 2013; Li Fazeng et al., 2016), and is an important parent of Sorbus germplasm innovation (Tang Wei et al., 2016). Due to the fact that the seedlings of Sorbus australis Beijing are grown, the progeny varies greatly; the rooting rate is low during vegetative propagation of cuttings, and mass propagation is difficult, which greatly affects the popularization and application. Tissue culture seedling technology is the key to...

AI Technical Summary

Problems solved by technology

Therefore, although the above-mentioned species and varieties with close or distant genetic relationship with Beijing Sorbus have a preliminary tissue culture rapid propagation technology system, the above-mentioned technical methods cannot be directly applied to Beijing Sorbus

[0004] Possibly due to technical difficulties such as the difficult propagation of Beijing Sorbus, at present only Xin Baoying (2013) has initially carried out a pioneering and beneficial exploration of the "micro-propagation technology" of Beijing Sorbus, but his research shows that with the use of related technologies, the lateral buds in the most ideal condi

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