A kind of tissue culture propagation method of resuscitated plant Selaginella mats

A technology for resuscitation of Selaginella mats, applied in the field of plant reproduction, can solve the problems of insufficiency, Selaginella mats are prone to browning, difficult to propagate by cuttings, etc., and achieves the effect of high reproduction efficiency

Active Publication Date: 2022-08-02
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, Selaginella cuspidatum has both ornamental and medicinal value, and wild resources have been extensively exploited, resulting in a sharp decline in the wild population of Selaginella cuspidatum, and wild resources can no longer meet the current demand
[0004] In the Selaginella genus, some species with creeping main stems, such as: Xiaocuiyun, have adventitious roots at the stem nodes, which can be propagated by cuttings, but the main stems of Selaginella cushions are not creeping, so it is extremely difficult to propagate by cuttings
In addition, it was found in the previous research that browning of Selaginella cuspidatum is very easy to occur during the tissue culture process, and the tissue culture propagation is difficult
At present, there is no report on the related propagation technology of the revived plant Selaginella cuspidatum, nor the tissue culture propagation method of the revived plant Selaginella cuspidatum

Method used

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  • A kind of tissue culture propagation method of resuscitated plant Selaginella mats
  • A kind of tissue culture propagation method of resuscitated plant Selaginella mats
  • A kind of tissue culture propagation method of resuscitated plant Selaginella mats

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1 The method of the present invention

[0028] (1) Obtaining sterile explants

[0029] Select 0.8-1.2 cm of the tip of the young shoots of wild Selaginella cuspidatum plants as explants, use a soft brush dipped in 1% v / v detergent water to clean the surface of the explants, and then place them in a beaker. Cover with gauze and rinse with running tap water for 2 hours. Under aseptic conditions, the explants were sterilized with a disinfectant for 12 minutes, and then rinsed with sterile water for 5 times, each rinse for 3 minutes, to obtain sterile explants. The disinfectant was HgCl at a concentration of 0.1% w / v 2 solution. The young shoots are the branches on the 2nd to 3rd layers outward from the central growth point of the Selaginella cuspidatum plant.

[0030] (2) Induction of primary buds

[0031] The sterile explants obtained in the step (1) were inoculated on the primary bud induction medium, placed in a 22° C. culture room for dark culture, and ...

Embodiment 2

[0039] Embodiment 2 and embodiment 3 are both methods of the present invention

[0040] Except for the different measures listed in Table 1, the other measures in Example 2 and Example 3 are the same as those in Example 1, and will not be repeated here.

[0041]Table 1 Examples 2 and 3 are different measures from the examples

[0042]

[0043] The reproductive effect of table 2 embodiment 1-3

[0044]

[0045] control

[0046] The control was the reproduction of wild plants of Selaginella cuspidatum in the wild habitat.

[0047] (1) Observation objects: In the same growing area in the wild, 30 2-3 year old wild plants of Selaginella cuspidatum with the same growth pattern were selected as the observation group.

[0048] (2) Wild habitat: bare limestone surface, extremely thin soil layer; distinct dry season and rainy season, the average annual precipitation is about 600-800 mm, of which the dry season precipitation accounts for 10-12%; the noon light intensity on a su...

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Abstract

A tissue culture propagation method for the resuscitated plant Selaginella serrata. The method includes: obtaining the sterile explants of Selaginella cuspidatum, inducing primary buds, multiplying adventitious buds, and cultivating tissue culture seedlings for strengthening and rooting. The invention successfully uses the tips of the young branches of the revived plant Selaginella cuscus as explants to construct a tissue culture propagation system of Selaginella cuspidatum, and the proliferation rate of adventitious buds is more than 8 times. The re-proliferation rate of a single adventitious bud is more than 11 times, and the tip of a young Selaginella pad can be propagated within 170 to 175 days to obtain more than 88 tissue culture seedlings, and a high reproduction efficiency is obtained. The large-scale propagation of Selaginella has laid a good foundation, which is of great significance to the protection of wild populations and the development and utilization of resources of the revived plant Selaginella serrata.

Description

technical field [0001] The invention belongs to the field of plant propagation, and in particular relates to a tissue culture propagation method for resuscitating a plant Selaginella mats. Background technique [0002] Cushion Selaginella [Selaginella pulvinata (Hook. & Grev) Maxim.], belonging to Selaginella family (Selaginellaceae) Selaginella genus, is cushion-shaped, the main stem is not prostrate, mainly growing on exposed limestone surfaces or stone crevices, The growing environment is arid. Selaginella cuspidatum is commonly known as "Nine Dead Resurrection Grass". It is a typical recovery plant. It has strong dehydration resistance and can maintain long-term survival through low metabolism under extreme dehydration. When the moisture conditions are suitable, it can quickly return to normal. grow. Selaginella cuscus is a special kind of ornamental plant resource because of its miraculous resuscitation properties. At the same time, Selaginella cuspidatum contains fl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 余蓉培汪国鲜浦艳飞李帆卢珍红莫锡君
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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