Efficient induction method of clerodendrum japonicum polyploidy

A polyploid, high-efficiency technology, applied in horticultural methods, botanical equipment and methods, individual particle analysis, etc., can solve the problems of high chimera rate, simultaneous doubling, cumbersome separation operations, etc., and achieve high mutagenesis efficiency and regeneration. The effect of short cycle and easy operation

Active Publication Date: 2022-04-12
GUANGDONG ACAD OF FORESTRY
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Problems solved by technology

However, there is no research report on the induction of polyploidy of tung tree at present, and the relevant technologies only involve suspension cell propagation technology (CN104255481 A) and tissue culture rapid propagation technology (CN111202002 A) etc.
In addition, the traditional use of seeds or stem buds as materials for polyploid induction cannot guarantee the synchronous doubling of chromosomes in somatic cells, resulting in a high rate of chimerism, cumbersome separation operations in the later stage, and low induction efficiency

Method used

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  • Efficient induction method of clerodendrum japonicum polyploidy
  • Efficient induction method of clerodendrum japonicum polyploidy
  • Efficient induction method of clerodendrum japonicum polyploidy

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A kind of high-efficiency induction method of polyploidy, comprising the following steps:

[0029] a. Pre-cultivation: Take the robust aseptic seedlings of Tungia tungii as the material, select the 3rd to 4th fully expanded leaves from top to bottom, cut the petiole with leaves as the induction material, inoculate it into the pretreatment medium, and carry out pre-cultivation . Described petiole with leaf: petiole is long 0.8-1cm and has 0.5-0.8cm 2 size of leaves. The pretreatment medium: each liter contains 2 mg of 6-BA, 0.05 mg of NAA, 30 g of sucrose and 9 g of agar, and the balance is MS medium, pH 5.8; culture conditions: temperature 23°C, dark culture for 5 days.

[0030] b. Polyploid induction: After the incision end of the petiole is slightly swollen, take out the whole leafy petiole and immerse it in an aqueous colchicine solution with a concentration of 20 mg / L to induce polyploidy. Induction culture conditions: temperature 25°C, culture in dark for 4h. T...

Embodiment 2

[0035] Except for the following differences, the method steps of this embodiment and embodiment 1 are the same; the difference is that the present embodiment uses the 5-6th sheet of aseptic seedlings of the tung tree to fully expand the leaves from top to bottom, and cut the leaves with leaves The petiole is the induction material, and the leafy petiole: the petiole is 1-1.5cm long and has a 2-3cm 2 size of leaves. The corresponding adventitious bud regeneration effects are shown in Table 1.

Embodiment 3

[0047] Except for the following differences, the method steps of this example and Example 1 are the same; the difference is that the concentration of NAA in the integrated medium for induction and differentiation used in step c is 1 mg / L; the corresponding callus induction rate and variable The bud regeneration effect is shown in Table 2.

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Abstract

The invention discloses an efficient induction method of clerodendrum japonicum polyploidy. According to the method, petioles with leaves in sterile seedlings of clerodendrum japonicum are directly used as induction materials, and the method comprises the steps of pre-culture, polyploidy induction, petiole adventitious bud regeneration and polyploidy flow cytometry. By utilizing the method, the problem of tissue culture browning of the clerodendrum japonicum caused by colchicine mutagenesis is solved, the callus induction and petiole adventitious bud regeneration process can be completed in one step, the tetraploid induction rate can reach up to 43.3%, and the method has the characteristics of simplicity in operation, low browning rate, short regeneration period, high mutagenesis efficiency and the like, and can provide technical support for creating new polyploid varieties of the clerodendrum japonicum.

Description

technical field [0001] The invention belongs to the technical field of plant polyploid breeding, and in particular relates to a high-efficiency induction method for the polyploid of the eucalyptus. Background technique [0002] Clerodendrum japonicum (Thunb.) Sweet, also known as Zhuangyuanhong, is a shrub plant of the genus Daqing in the Verbenaceae family, and is widely distributed in southern provinces of my country. The tung tree is a traditional wild flower in my country. Its flower color is as bright as fire, and the annual flowering period lasts for 6 months. It has the reputation of "hundred-day red" and is deeply loved by the people of Lingnan. At the same time, Tongtong is a traditional Chinese medicinal material in the Lingnan area. The whole plant is used medicinally, and it is included in "Chinese Materia Medica". In addition, Tung tree is an extremely shade-tolerant shrub plant that can grow in woodlands with a canopy density as high as 0.8 without excessive t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00G01N15/14
Inventor 苏凌业王洪峰何春梅王丛丛徐明锋庄赟廖桂花
Owner GUANGDONG ACAD OF FORESTRY
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