Plant source domestic poultry feed antibacterial additive and preparation method thereof

An antibacterial additive and plant-sourced technology, applied in animal feed, animal feed, plant raw materials, etc., can solve the problem of thyme essential oil affecting antibacterial effect, improve palatability and gastrointestinal function, novel ideas, and improved stability Effect

Active Publication Date: 2018-01-05
哈尔滨吉粮农牧科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented new technology allows create plants that have specific qualities such as being safe from harmful microorganisms or having beneficial characteristics like enhancing nutritional value (favaal protein). These products could be useful in making better ways of producing food without adding any chemical ingredients they may contain.

Problems solved by technology

This patents discuss different methods for modifying plants' physicochemistry and nutritional value while also addressing issues related to environmental safety concerns associated with pesticides including synthetic detergents like tristezolidines and chloroquaternary ammoniums. Additionally, there may exist potential risks posed during handling processes involving these agents themselves, especially through their release overtime. To overcome this issue, the patented proposals involve adding therapy adjuvants containing specific ingredients called phytate microbons, specifically lithochrysanthemum and lycopersma at very small sizes. These adjuncts help enhance the activity of drugs target gut cells without causing unwanted side affects.

Method used

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  • Plant source domestic poultry feed antibacterial additive and preparation method thereof
  • Plant source domestic poultry feed antibacterial additive and preparation method thereof
  • Plant source domestic poultry feed antibacterial additive and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Poultry Feed Antibacterial Additive—Preparation of Thyme Essential Oil Solid Liposome

[0039] 1 Experimental materials

[0040]

[0041] 2 experimental equipment

[0042]

[0043] 3 Experimental methods

[0044] ① Put soybean lecithin and cholesterol (5:1, w / w) in a round bottom flask, add a certain amount of thyme essential oil (5.0mg / mL) and organic solvent chloroform (50.0mL), ultrasonically disperse (100Hz, 30min ) to make it fully dispersed and dissolved.

[0045] ②Use a rotary evaporator (30-40°C) to evaporate the organic solvent until a smooth light yellow lipid film is formed on the inner wall of the round bottom flask. Then put the lipid film into a vacuum drying oven, dry it at 60° C. for 24 hours in a vacuum state, and then take it out. Surfactant PVP (1.0 mg / mL), cryoprotectant mannitol (1.0 mg / mL) and PBS (50.0 mL) were added, and the water bath was ultrasonically mixed (100 Hz, 3 h) until a stable emulsion was formed.

[0046] ③Cru...

Embodiment 2

[0050] Embodiment 2 Poultry Feed Antibacterial Additive—Characterization of Thyme Essential Oil Solid Liposome

[0051] 1 Experimental materials

[0052] Thyme essential oil solid liposome

[0053] 2 experimental equipment

[0054]

[0055] 3 Experimental methods

[0056] ①The particle size, polydispersity index (PDI) and Zeta potential of thyme essential oil solid liposomes were determined by laser particle size analyzer. The samples were diluted 10 to 20 times with PBS for measurement. The parameters of thyme essential oil nanoliposomes (emulsion state) without ε-PL modification were also determined as a control.

[0057] ② Encapsulation efficiency (%) and drug loading (%) were calculated by GC-MS analysis.

[0058] a. First draw the standard curve: Thyme essential oil was diluted with absolute ethanol to a standard solution with a concentration of 0mg / mL, 0.2mg / mL, 0.4mg / mL, 0.6mg / mL, and 0.8mg / mL, and passed GC- MS analysis, drawing a standard curve.

[0059] b. ...

Embodiment 3

[0067] Example 3 Poultry Feed Antibacterial Additive—Release of Thyme Essential Oil in Chicken Simulated Gastric Juice in Thyme Essential Oil Solid Liposome

[0068] 1 Experimental materials

[0069] Thyme essential oil solid liposome and thyme essential oil solid liposome-plasma

[0070] Chicken simulated gastric juice composition: pepsin: 3.0mg / mL

[0071] Distilled water: 100mL

[0072] pH value: 2.0~3.5 (use 1.0mol / L hydrochloric acid to adjust pH)

[0073] Filter through a bacterial filter and set aside.

[0074] 2 experimental equipment

[0075]

[0076] 3 Experimental methods

[0077] In order to better apply the product in practice, we choose chicken, which is common in poultry, as a model poultry animal for experimental analysis. A certain amount of thyme essential oil solid liposome-plasma was weighed into 50.0mL chicken simulated gastric juice, and placed in a constant temperature water bath at 40°C (simulated chicken body temperature) for cultivation. At ...

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Abstract

The present invention belongs to the field of feed antibacterial additives and particularly relates to a plant source domestic poultry feed antibacterial additive and a preparation method thereof. Thyme essential oil is used as a main antibacterial agent to prepare thyme essential oil nano-liposomes, epsilon-polylysine (epsilon-PL) having an antibacterial property is used to conduct a modificationof surfaces of the nano-liposomes, a freeze-drying method is used to transfer the thyme essential oil nano-liposomes into solid liposomes, then finally low-temperature plasmas are utilized to conducta surface treatment modification of the solid liposomes, and an antibacterial effect is further improved. The related plant source domestic poultry feed antibacterial additive is simple in preparation processes, good in product stability, uniform in particle size distribution, and relatively high in encapsulation efficiency and drug loading capacity, and has remarkable antibacterial effects on four commonly-occurring susceptible pathogens in domestic poultry.

Description

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Claims

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Application Information

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Owner 哈尔滨吉粮农牧科技有限公司
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