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Method for simply and rapidly screening sorghum varieties with high emergence rate

A technology of emergence rate and sorghum, which is applied in the field of crop genetics and breeding, can solve the problems of slow emergence, fast emergence, and high emergence rate, and achieve the effects of short cycle, improved work efficiency, and simple operation

Inactive Publication Date: 2018-01-09
JILIN ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

For materials with long mesocotyls, the hypocotyl elongation is obvious, the emergence speed is fast, and the emergence rate is high; while for materials with short mesocotyls, the emergence speed is slow and the emergence rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the method for cultivating sorghum mesocotyl

[0051] 1. Germination

[0052] For each batch of materials, 100 seeds of the same size and full grains were selected, sterilized with 1g / L mercuric chloride solution for 10 minutes, and then washed 3 times with tap water and 3 times with deionized water. Arrange evenly and neatly in a 9cm petri dish with a lid covered with 2 layers of filter paper, 50 seeds per petri dish, add 10ml of water, 2 petri dishes for each material. Put it into an incubator at a temperature of 30°C and incubate in the dark for 24 hours.

[0053] 2. Culturing Mesocotyls

[0054] Take out the petri dish that has been cultivated in the dark for 24 hours, and select the seeds that have just emerged and have the same size for each material, and put them into a culture box with a cover. 20 germinated seeds were placed on filter paper, and 10ml of water was added to each culture box. Each material was repeated 3 times. Put them into an ...

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Abstract

The invention provides a method for simply and rapidly screening sorghum varieties with high emergence rate and in particular discloses a method for simply and rapidly determining elongation of mesocotyl in a sorghum variety, parent or coeno-species. According to the method, seeds subjected to accelerating germination are cultured in dark by utilizing a water culture box with filter paper, the obtained mesocotyl in the sorghum is in an upright state, convenience is brought to subsequent measurement, or the mesocotyl can be directly visually observed and compared, the measurement is completelysaved, and the operating efficiency of the conventional actual onerous operation for screening and measuring the mesocotyl in the sorghum is greatly improved. In addition, due to the water culture andthe method for culturing the mesocotyl in the sorghum in dark, the cycle is short, and the operation is simple, so that relative emergence rate conditions of the sorghum varieties with different genetic backgrounds can be efficiently judged or compared.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, in particular to a method for screening sorghum varieties with high emergence rate. Background technique [0002] The low germination rate of sorghum seeds is a common problem in production, which often causes great losses to agricultural production. There are many reasons for the low germination rate of sorghum seeds, such as climatic factors (low temperature damage), farming practices, sowing depth, pests and diseases, etc., but the maximum potential of sorghum seed germination ability is determined by genetics. There are extremely significant differences in length, days of emergence and emergence rate. Screening sorghum varieties with high emergence rate is a very necessary work for sorghum production and planting and sorghum genetics and breeding research. The method for high sorghum varieties can greatly improve the work efficiency of sorghum production and planting and sorghum gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01C1/00A01C1/08A01G31/00
Inventor 陈冰嬬徐宁石贵山王鼐于淼侯佳贤杨静高士杰
Owner JILIN ACAD OF AGRI SCI
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