High-variation rate breeding method for peony

A technology of mutation rate and peony, applied in seed immunization, seed and rhizome treatment, application, etc., can solve the problems of high cultivation cost, low mutation probability, slow cultivation of new varieties, etc., to avoid degradation, reduce damage, improve Effects of nutrient accumulation and cell activity

Inactive Publication Date: 2018-01-26
当涂县海纳农业科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention is aimed at the existing problems: traditional breeding produces new varieties through hybridization or natural variation, the variation probability is small, the cultivation speed of new varieties is slow, and the cultivation cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for breeding peony with high variation rate, comprising the following steps:

[0018] (1) Seed storage: Sterilize peony seeds to avoid degradation of seeds by pathogenic bacteria and reduce seed activity, and then immerse in nutrient solution for low-temperature storage. The culture solution is rich in organic selenium, which can improve the self-protection ability of seeds and reduce The damage caused by irradiation and chemical mutagenesis can improve the survival rate of the mutant seeds, and the nutrient solution is rich in amino acids, plant growth-promoting factors and other components can improve the nutrient accumulation and cell activity of the seeds, first store at 8.5°C for 3.5 h, then lower the temperature to 2.5°C and store for 13 days to store the seeds;

[0019] (2) Vacuum mutagenesis: first immerse the stored seeds in the germination solution for 2.5 hours to improve seed activity and differentiation ability, and then immerse in the colchicine s...

Embodiment 2

[0028](1) Seed storage: Sterilize peony seeds to avoid degradation of seeds by pathogenic bacteria and reduce seed activity, and then immerse in nutrient solution for low-temperature storage. The nutrient solution is rich in organic selenium, which can improve the self-protection ability of seeds and reduce The damage caused by irradiation and chemical mutagenesis can improve the survival rate of the mutant seeds, and the nutrient solution is rich in amino acids, plant growth-promoting factors and other ingredients can improve the nutrient accumulation and cell activity of the seeds, first store at 9.5°C for 4 hours , then lower the temperature to 3.5°C and store for 14 days to store the seeds;

[0029] (2) Vacuum mutagenesis: first immerse the stored seeds in the germination solution for 3 hours to improve the seed activity and differentiation ability, and then immerse in the colchicine solution in a vacuum tank for mutation breeding. The gravitational resistance is small, an...

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PUM

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Abstract

The invention discloses a high-variation rate breeding method for peony. The method comprises the following steps: 1) seeds storage, performing sterilization on peony seeds and immersing the peony seeds in a nutrient solution, and performing low-temperature storage; 2) vacuum mutagenesis, immersing the stored seeds in germination accelerating liquid, then immersing the material in a colchicine solution in a vacuum tank and respectively using gamma-ray and ultraviolet ray for alternation for breeding; 3) germination accelerating at constant temperature, immersing the mutagenesis seeds in an organic selenium solution, and then immersing the material in a nutrient solution for germination accelerating at constant temperature; and 4) seedling growing, sowing the seeds in a seedling growing case, regularly sprinkling the nutrient solution, and adjusting carbon dioxide concentration for seedling growing. Compared with a control group, the mortality after seed mutagenesis is reduced by 20.46%, the variation rate is increased by 11.49%, the type quantity is increased by 5, and the florescence time of new species of peony is prolonged by 11 days.

Description

technical field [0001] The invention belongs to the technical field of peony biological breeding, and in particular relates to a method for breeding peony with high variation rate. Background technique [0002] Peony is a plant of Ranunculaceae and Paeonia genus. It is a perennial deciduous shrub. Its stem grows up to 2 meters, and its branches are short and thick. In the Tang Dynasty, there was a poem praising peony, "the peony in front of the court is invincible, and the lotus on the pond is pure and unfeeling. Only the peony is really beautiful, and the flowers move the capital when it blooms." It is popular in China. Occupy an important position in traditional cultural poetry and painting. There are many varieties of peonies, among which yellow, green, flesh red, deep red and silver red are the top grades, especially the yellow and green varieties are the most precious. The cultivar of peony is produced through its hybridization or natural variation, and then it is cul...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/08
Inventor胡晨
Owner当涂县海纳农业科技有限公司