Method used for evaluating health of hermatypic coral and based on activity of caspase3

A coral and reef technology, applied in the field of biological health assessment, can solve the problem of high mortality in artificial coral transplantation, and achieve the effect of simple and fast operation process

Active Publication Date: 2018-02-02
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the selection of transplant mothers, transplant environment, transplant methods, and post-transplant management and maintenance, the mortality rate of artificial coral transplantation remains high.

Method used

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  • Method used for evaluating health of hermatypic coral and based on activity of caspase3
  • Method used for evaluating health of hermatypic coral and based on activity of caspase3
  • Method used for evaluating health of hermatypic coral and based on activity of caspase3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Staghorn cup coral ( Pocillopora damicornis ) health status assessment as an example, including the following three steps:

[0031] (1) Select a total of 25 samples of Pocillopora staghorn in ideal growth state and 32°C high temperature stress samples, wash some coral tissues with seawater filtered through a 0.22 microporous membrane, centrifuge at 1500 g at 4°C for 15 min, The supernatant of the washing liquid was taken and stored in a -80°C ultra-low temperature refrigerator for caspase3 activity determination. The caspase3 activity was measured using KGI Biocaspase3 Spectrophotometric Detection Kit;

[0032] (2) Take the same batch of samples and artificially transplant other corals into a suitable growth environment to observe the growth;

[0033] (3) Based on the data of caspase3 activity and coral survival rate after transplantation, establish the health evaluation standard of Pocillopora staghorn coral.

Embodiment 2

[0035] Staghorn cup coral ( P. damicornis ) health status assessment as an example, including the following three steps:

[0036] (1) Select the Pocillopora staghorn coral under the ideal growth state and receive 0.01 mol L -1 NH 4 + A total of 35 samples of Pocillopora staghorn coral under ammonia enrichment stress were rinsed with seawater filtered through a 0.22 microporous membrane, centrifuged at 1500 g at 4 °C for 15 min, and the supernatant of the washing liquid was stored in a -80 °C ultra-low temperature refrigerator. Determination of caspase3 activity. The caspase3 activity was measured using KGI Biocaspase3 Spectrophotometric Detection Kit;

[0037] (2) Take the same batch of samples and artificially transplant other corals into a suitable growth environment to observe the growth;

[0038] (3) Based on the data of caspase3 activity and coral survival rate after transplantation, establish the health evaluation standard of Pocillopora staghorn coral.

Embodiment 3

[0040] Taking the assessment of the health status of two different reef-building corals in the Wenchang sea area of ​​Hainan as an example, the following two steps are involved:

[0041] (1) Select the samples of clustered helmeted coral and truncated rose coral in different growth states, divide them into 3 groups according to different growth environments, and each group has 6 biological replicates, and use the KGI biological caspase3 spectrophotometric detection kit to detect Coral cell caspase3 activity;

[0042] (2) Judging the health status of corals based on caspase3 activity data and growth status.

[0043] The data of three embodiments of the present invention are as follows:

[0044] Example

healthy

Sub-health

damaged or albino

1

1.00-1.05

1.14-1.28

>1.36

2

1.00-1.05

1.22-1.35

>1.43

[0045] Coral Health

survival rate

healthy

100%-95%

Sub-health

50%-30%

damaged or albino...

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Abstract

The invention provides a method used for evaluating health of hermatypic coral and based on activity of caspase3. The method includes the steps of: determining a biological index, selecting standard reference points and establishing an evaluation standard, wherein the step of determining the biological index comprises the steps of: selecting the key enzyme caspase3, which is used for responding the environmental stress, of the hermatypic coral, and adopting the activity of the caspase3 of the hermatypic coral as the index for evaluating the health level of the coral, and the step of selectingthe standard reference points comprises that coral samples in ideal growing states and under different stress conditions are selected as the reference points; the step of establishing the evaluation standard comprises that the coral health level standard is established according to data about the activity of the caspase3 of different reference point samples, the growing states of the hermatypic coral and the survival rate of the coral in an growing environment which is restored to an ideal seawater environment. According to the method used for evaluating the health of the hermatypic coral andbased on the activity of the caspase3, the health level of the coral can be evaluated objectively and accurately, and the operation process is simple and fast; besides, not only can the blank of the technology for the investigation of coral resources be filled, but also the selection of a parent body can be guided during artificial transplantation of the coral, so that the research and protectionof the coral reef ecosystem are facilitated.

Description

technical field [0001] The invention relates to the field of biological health assessment, in particular to a method for assessing the health level of reef-building corals, which is applied to the protection and restoration of coral reef ecosystems. Background technique [0002] Coral reefs are one of the typical ecosystems with the richest biodiversity and the highest productivity. Known as the "tropical rainforest in the ocean", they have extremely high primary productivity and play an irreplaceable role in regulating the global climate and maintaining the balance of the ecosystem. . Coral reefs in the world are mainly distributed in tropical and subtropical seas. About 110 countries around the world have coral reef resources, and their total area (617,000 km 2 ) occupies about the entire sea area (340×10 6 km 2 ), and recorded reef-dwelling organisms account for 30% of the total number of marine organisms. The coral reef ecosystem not only provides people with food, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 周智俞小鹏唐佳吴逸波赵水苗苏逸璐王佳俊黄玉连黄勃
Owner HAINAN UNIVERSITY
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