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Technology for extracting and purifying dendrobium polysaccharides of dendrobium officinale root

A technology for Dendrobium officinale and Dendrobium polysaccharide, which is applied in the field of deep processing of pharmaceutical raw materials, can solve problems such as waste waste, and achieve the effects of low production cost, small solvent consumption and high product quality

Inactive Publication Date: 2018-02-13
NANJING XIANCAOTANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, previous studies on Dendrobium officinale have mainly focused on its main stem, and there are many and in-depth reports in related documents, while there are few or shallow research reports on its fibrous roots, scale leaves and flowers. Sometimes wasted as waste disposal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: A kind of extraction and purification process of Dendrobium officinale polysaccharides

[0027] A process for extracting and purifying polysaccharides from dendrobium candidum root, said process comprising the following steps:

[0028] (1) Collect fresh Dendrobium candidum, take the roots, wash, dry, and store in a cool place for later use; immerse the treated fresh Dendrobium candidum roots in 5 times the volume of acetic acid solution for 1 hour, then wash them with water, and dry them in the air; The acetic acid solution is an aqueous acetic acid solution with a volume fraction of 36%, and in other embodiments, an aqueous acetic acid solution with a volume fraction of 10-98% can be used.

[0029] (2) Submerge the dried root in step (1) in 3 times the ethanol with a volume fraction of 75%, reflux extraction twice, each time for 1 hour, filter, collect filter residue, wash with water, and dry to obtain the medicine scum.

[0030] (3) Powder the medicinal d...

Embodiment 2

[0034] Example 2: A kind of extraction and purification process of Dendrobium officinale polysaccharides

[0035] A process for extracting and purifying polysaccharides from dendrobium candidum root, said process comprising the following steps:

[0036] (1) Collect fresh Dendrobium candidum, take the root, wash, dry, and store in a cool place for later use; immerse the treated fresh Dendrobium candidum root in 8 times the volume of acetic acid solution for 2.5 hours, then wash it with water, and dry it; The acetic acid solution is an aqueous acetic acid solution with a volume fraction of 38%, and in other embodiments, an aqueous acetic acid solution with a volume fraction of 10-98% can be used.

[0037] (2) Submerge the dried root in step (1) in 12 times the ethanol with a volume fraction of 80%, reflux extraction 4 times, 2 hours each time, filter, collect filter residue, wash with water, and dry to obtain the drug scum.

[0038] (3) Powder the medicinal dregs collected in ...

Embodiment 3

[0042] Example 3: A kind of extraction and purification process of Dendrobium officinale polysaccharides

[0043] A process for extracting and purifying polysaccharides from dendrobium candidum root, said process comprising the following steps:

[0044](1) Collect fresh Dendrobium candidum, take the root, wash, dry, and store in a cool place for later use; immerse the treated fresh Dendrobium candidum root in 8 times the volume of acetic acid solution for 2 hours, then wash it with water, and dry it; The acetic acid solution is an aqueous acetic acid solution with a volume fraction of 45%, and in other embodiments, an aqueous acetic acid solution with a volume fraction of 10-98% can be used.

[0045] (2) Submerge the dried root in step (1) in 12 times the ethanol with a volume fraction of 85%, reflux extraction 4 times, 1 hour each time, filter, collect filter residue, wash with water, and dry to obtain the drug scum.

[0046] (3) Powder the medicinal dregs collected in step...

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PUM

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Abstract

The invention discloses a technology for extracting and purifying dendrobium polysaccharides of a dendrobium officinale root. The technology comprises the following steps: soaking the root in an acetic acid solution; cleaning and airing and then soaking in ethanol; performing reflux extraction, filtering and collecting filter residue; cleaning and airing to obtain medicine residue; grinding the medicine residue and performing water-bath reflux extraction; filtering the extract solution to obtain filtrate; adding absolute ethyl alcohol into the filtrate; standing overnight, then centrifuging and taking the precipitate; airing the precipitate and dissolving in deionized water; adding a Sevage reagent; performing operations of vibration, standing and centrifuging in turn; repeating 3-5 times,and combining the collected upper-layer solution; adding activated carbon into the upper solution; stirring and then filtering; performing reduced-pressure concentration of the filtrate to obtain thedendrobium polysaccharides of dendrobium officinale root. The technology disclosed by the invention not only can protect the active ingredients of dendrobium officinale, but also maintains the structure of dendrobium polysaccharides to the greatest extent.

Description

technical field [0001] The invention relates to an extraction and purification process of active ingredients, in particular to an extraction and purification process of dendrobium polysaccharides from the root of dendrobium officinale, and belongs to the technical field of deep processing of medicinal raw materials. Background technique [0002] Dendrobium candidum is a perennial herbaceous plant of the family Dendrobium. Its main medicinal parts are fresh or dried stems. It is mainly distributed in Anhui, Zhejiang, Guangxi, Hunan, Yunnan, Guizhou and other places. It is a traditional precious Chinese medicinal material. Dendrobium officinale contains dendrobium polysaccharides, flavonoids, proteins, 17 kinds of amino acids and inorganic elements, etc. It has the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. "Shen Nong's Materia Medica" listed it as the top grade, and the Taoist health classic "Tao Zang" praised it as the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 史月龙曾宋君吴英亮刘文斌吴坤林汤静
Owner NANJING XIANCAOTANG BIOLOGICAL TECH CO LTD
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