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Special culture medium for cypripedium palangshanense

A culture medium, benzyl aminopurine technology, applied in the field of special medium for Balangshan almonds, can solve problems such as scarcity and extinction, and achieve the goal of inhibiting the growth of stems and leaves, ensuring water balance, and improving survival rate and rooting rate. Effect

Inactive Publication Date: 2018-02-27
陶象余
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the latest discoveries in recent years, Balangshan Cypedium is a medicinal plant with high medicinal value, but due to its special growth environment and conditions, its number is scarce and it is facing the crisis of extinction. Therefore, the development of It is very necessary to develop a medium for Cypripedium balangshan for artificial cultivation

Method used

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  • Special culture medium for cypripedium palangshanense
  • Special culture medium for cypripedium palangshanense

Examples

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Embodiment 1

[0012] A special culture medium for Cypripedium in Balang Mountain, including the following components: 1 / 2MS medium, 0.9mg / L humus, 1.2mg / L indolebutyric acid, 0.7mg / L bijiu, 0.5mg / L6-benzyl Aminopurine, 0.08mg / L euconazole, 0.2mg / L triiodobenzoic acid, 1mg / L brassinolide, 2g / L white sugar, 6g / L agar, the pH value of the medium is 6- 7.

Embodiment 2

[0014] A special culture medium for Cypripedium balangshan, including the following components: 1 / 2MS medium, 1.5mg / L humus, 0.8mg / L indolebutyric acid, 0.9mg / L bijiu, 1mg / L6-benzyl Aminopurine, 0.3mg / L euconazole, 0.5mg / L triiodobenzoic acid, 0.6mg / L brassinolide, 5g / L white granulated sugar, 10g / L agar, the pH value of described culture medium is 6- 7.

Embodiment 3

[0016] A special culture medium for Cypripedium balangshan, including the following components: 1 / 2MS medium, 1.2mg / L humus, 1mg / L indolebutyric acid, 0.8mg / L bijiu, 0.6mg / L6-benzyl Aminopurine, 0.2mg / L euconazole, 0.3mg / L triiodobenzoic acid, 0.9mg / L brassinolide, 3g / L white sugar, 8g / L agar, the pH value of the medium is 6-7.

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Abstract

The invention discloses a special culture medium for Cypripedium balangshan, which comprises the following components: 1 / 2MS culture medium, 0.9-1.5mg / L humus, 0.8-1.2mg / L indole butyric acid, 0.7-0.9mg / L L Bijiu, 0.5‑1mg / L6-benzylaminopurine, 0.08‑0.3mg / L Euconazole, 0.2‑0.5mg / L Triiodobenzoic acid, 0.6‑1mg / L Brassinolide, 2‑5g / L L white granulated sugar, 6-10g / L agar, the pH value of described culture medium is 6-7. The present invention adopts commonly used MS culture medium as base, has added the humus that promotes the growth of Balangshan Cypripedium, indolebutyric acid, 6-benzylaminopurine, and euconazole and triiodobenzoic acid that promote root growth, and can Provide a suitable living environment for Balangshan Cypripedium, ensure water balance, and effectively improve the survival rate and rooting rate of Balangshan Cypedopsis.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a special culture medium for Cypripedium balangshan. Background technique [0002] Cypripedium palangshanense Tang&F.T.Wang (scientific name: Cypripedium palangshanense Tang&F.T.Wang) is produced from the west to the southwest of Sichuan, my country, and is born in the forest or bush at an altitude of 2200-2700 meters. Its plants are 8-13 cm tall, with slender and transverse rhizomes. Stems erect, glabrous, mostly enclosed in several sheaths, with 2 leaves at the top. Leaves opposite or nearly opposite, flat; blades nearly round or nearly broadly elliptic. Inflorescence terminal, suberect, with 1 flower; flowers drooping, blood red or lavender; petals obliquely lanceolate, 1.2-1.6 cm long, 4-5 mm wide, apex acuminate, back base slightly hairy; Lip sac-shaped, subspherical, about 1 cm long, with a broad, nearly round sac opening; staminodes ovate-lanceolate, about 3 mm long. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 陶象余
Owner 陶象余
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