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Targeting inhibitor of RAX2 (Retina and Anterior Neural Fold Homeobox 2) gene and purpose thereof

An inhibitor and targeted technology, applied in the field of medicine, can solve the problems that the median survival time is only 12 months, the survival rate is less than 13%, and the application of RAX2 is blank. The effect of drug resistance

Inactive Publication Date: 2018-03-06
SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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Problems solved by technology

However, because gliomas have the biological characteristics of invasive growth, and the higher the grade of gliomas, the stronger the invasiveness, even if the surgery achieves total resection under imaging and microscopy, recurrence is still inevitable
The current mainstream surgery plus concurrent radiotherapy after surgery shows that the median survival time is only 12 months, and the 5-year survival rate is less than 13%.
[0005] At present, the role of RAX2 in the development of glioma and related mechanisms have not been reported, and the application of RAX2 in glioma gene therapy is still blank.

Method used

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  • Targeting inhibitor of RAX2 (Retina and Anterior Neural Fold Homeobox 2) gene and purpose thereof
  • Targeting inhibitor of RAX2 (Retina and Anterior Neural Fold Homeobox 2) gene and purpose thereof
  • Targeting inhibitor of RAX2 (Retina and Anterior Neural Fold Homeobox 2) gene and purpose thereof

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Experimental program
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Embodiment 1

[0043] 1. Preparation of glioma cells of different grades (I\II\III\IV)

[0044] Human glioblastoma cell lines U87 and U251 were purchased from the Life Science Cell Resource Center of Shanghai Research Institute, and the medium used was DMEM high-glucose medium containing 10% serum, 100 U / ml penicillin and 100 µg / ml streptomycin Or F12 high-sugar culture medium, and the culture environment is a 37°C constant temperature incubator containing 5% CO2 and humidity.

[0045] 2. Real-time quantitative PCR detection of the expression level of RAX2 gene in normal brain tissue and different grades of glioma tissue.

[0046] (1) Trizol method was used to extract total RNA from cells.

[0047] Wash the collected cells with cold PBS, add 1 ml Trizol reagent and pipette several times, observe under the microscope that the cells are in the shape of oil droplets (fully lysed), then transfer to a 1.5 ml EP tube, and let it stand for 5 minutes to fully lyse; Add 0.2ml of chloroform to th...

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a targeting inhibitor of an RAX2 (Retina and Anterior Neural Fold Homeobox 2) gene and a purpose thereof. According to the targeting inhibitor of the RAX2 gene, the gene sequence of the targeting inhibitor is 5'-GGAGGAACCGCACCACCTTCATTCAAGAGA-3' (SEQ ID No. 1). The inhibitor can be specifically bound with the RAX2 gene; the RAX2 gene is silenced; thus, the influences, on the cell proliferation, migration and invasion ability of a glioma, of an RAX2 are inhibited; the cell apoptosis of the glioma is induced and the goal of treating the glioma is realized.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a targeted inhibitor of RAX2 gene and its application. Background technique [0002] Glioma is the most common malignant tumor of the central nervous system, accounting for 50%-60% of primary brain tumors. Currently, the main treatment methods are surgery, with chemotherapy and radiotherapy as adjuvant therapy. However, because gliomas have the biological characteristics of invasive growth, and the higher the grade of gliomas, the stronger the invasiveness, even if the surgery achieves total resection under imaging and microscopy, recurrence is still inevitable. The current mainstream surgery plus concurrent postoperative radiotherapy statistics show that the median survival time is only 12 months, and the 5-year survival rate is less than 13%. The prognosis of high-grade gliomas is even worse. For example, the curative effect of glioblastoma multiforme (GBMs) is...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K31/713A61P35/00
CPCA61K31/713
Inventor 刘云会薛一雪蔡恒刘啸白郑健巩威李振赫倩茹马珺刘丽波王萍李志清王振华商秀丽苏瑞
Owner SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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