A method for cultivating regenerated seedlings of hybrid poplar and populus euphratica

A cultivating method and hybrid technology are applied in the field of cultivating regenerative seedlings of poplar and poplar hybrids, which can solve problems such as no reports and achieve the effect of improving the speed of seedling formation.

Active Publication Date: 2020-06-16
GANSU DESERT CONTROL RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, foreign research reports on Populus euphratica are still blank. Domestic scholars have mainly carried out some research on hybrid breeding technology, hardwood cutting propagation, introduction and domestication, and drought resistance of seedlings. See the report

Method used

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  • A method for cultivating regenerated seedlings of hybrid poplar and populus euphratica
  • A method for cultivating regenerated seedlings of hybrid poplar and populus euphratica
  • A method for cultivating regenerated seedlings of hybrid poplar and populus euphratica

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Embodiment Construction

[0031] The present invention is described in detail below in conjunction with accompanying drawing and specific embodiment:

[0032] Such as figure 1 The cultivation method of the shown Populus microphylla and Populus euphratica hybrid seedling comprises,

[0033] 1. Establish a sterile system

[0034] 1.1 Processing of explant material

[0035] Cut the 20-30cm long branches of Populus euphratica grown outdoors, bring them back to the laboratory, use a soft brush to dip in tap water with detergent to clean the stains and dust on the surface of the explant branches, and then dip them in with a glass rod Take a drop of Tween 80 for deep cleaning. After cleaning, rinse the explants under running water for 4 to 6 hours, and then place them on an ultra-clean workbench for sterilization. The specific sterilization time setting and sterilization test are as follows: adopt the method of orthogonal test to arrange the experimental content, the first step is to sterilize the explants...

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Abstract

The invention discloses a cultivation method for regeneration seedlings of a hybrid of populus simonii and populus euphratica. The cultivation method is characterized by comprising the following steps: establishment of an explant sterilizing and sterility system, adventitious bud induction and proliferation, rooting culture and acclimatization and transplantation. According to the invention, by adopting a tissue culturing method, rapid propagation of populus simonii and populus euphratica high-quality test-tube plantlet is realized within relatively short time, and only about 60 days are needed from cluster buds induction and proliferation to test-tube plantlet rooting in the whole propagation process, and thus greatly increasing the plantlet formation speed of populus simonii and populuseuphratica, having important functions for the large-scale and industrialized production and propagation of plants of populus simonii and populus euphratica, and continuously supplying high-quality nursery stocks for saline land reclamation of the dry desert region and afforestation in the desert region in the northwest of China.

Description

Technical field: [0001] The invention belongs to the technical field of plant regenerated seedling cultivation, and in particular relates to a method for cultivating regenerated seedlings of hybrid poplar and populus euphratica. Background technique: [0002] There is a large area of ​​desertification and salinization in the northwest of my country. The improvement and utilization of these lands is an important task for the construction of the ecological environment and the sustainable development of forestry in my country. The lack of tree species for afforestation is the main difficulty in improving desert salinization land. One, in order to solve this problem quickly and effectively, in addition to the introduction and domestication of salt-tolerant tree species, it is also necessary to vigorously carry out the selection and breeding of salt-tolerant and drought-tolerant varieties. [0003] Populus simonii×P.euphratica (Populus simonii×P.euphratica) is a new poplar hybrid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02A40/22Y02P60/40
Inventor 王方琳尉秋实柴成武王飞
Owner GANSU DESERT CONTROL RES INST
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