A kind of rooting method of adventitious buds outside the bottle of Tissue Culture of Lycium ruthenicum

Abstract

The invention provides a lycium ruthenicumtissue culture adventitious bud ex-vitro rooting method, which belongs to the technical field of lycium ruthenicum tissue culture. The method comprises the following steps of (1) culturing lycium ruthenicum stem sections into an adventitious bud culture medium to obtain an adventitious bud; (2) directly hardening the adventitious bud obtained in the adventitious bud culture medium to obtain an adventitious bud with a root primordium; feeding a rooting powder solution into the adventitious bud culture medium during a seedling hardening process; (3) sterilizing the adventitious bud with the root primordium, soaking a root part through an IBA solution, transplanting into a substrate, and culturing to obtain a lycium ruthenicum grown-up seedling. The method is simple and convenient to operate and low in culture cost; the whole production cost is reduced by not less than 20 percent; the method is more suitable for mass industrialized production andvariety breeding of lycium ruthenicum seedlings, lays a solid foundation for building a lycium ruthenicum asexual rapid propagation system and providing seedlings for ecological environment construction in northwest China, and has better economic benefit, social benefit and ecological benefit.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of Lycium barbarum black fruit, and in particular relates to a method for rooting out of a bottle of adventitious buds of Lycium barbarum black fruit tissue culture. Background technique [0002] Black fruit wolfberry (Lycium ruthencium), belonging to the genus Lycium ruthencium of Solanaceae, is a thorny shrub, multi-branched, hard branches, often zigzag curved, white. Black fruit Lycium barbarum is distributed in Qinghai, Ningxia, Xinjiang, Gansu and other places in China and Europe and Central Asia. It is a typical desert plant and has strong adaptability to environmental conditions. It has the characteristics of drought resistance, cold resistance, barren resistance, and salt-alkali tolerance. [0003] Lycium barbarum is an important economic plant and a good product for both medicine and food. Its fruit can be used as medicine, which has the effects of enhancing immunity, delaying agi...

AI Technical Summary

Problems solved by technology

At present, Lycium barbarum is mainly propagated by seeds, and there are also a large number of research reports on asexual cutting propagation and tissue culture test tube seedlings of Lycium barbarum. Although asexual cutting propagation can become seedlings after the cuttings take root, it cannot be multiplied. The problem of slow speed; however, in the conventional tissue culture propagation process, there are many problems in the cultivation process, slow growth of seedlings, and high cost

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