Method for detecting content of marbofloxacin in dog blood plasma

Abstract

The invention discloses a method for detecting the content of marbofloxacin in dog blood plasma. The method comprises the following steps: 1, taking a marbofloxacin-containing dog blood plasma sample,adding marbofloxacin used as an internal standard substance, carrying out vortex uniform mixing for 8-13 s, adding acetonitrile to precipitate proteins, carrying out vortex uniform mixing for 20-40 s, standing the obtained material for 2-4 min, centrifuging the material, and taking the obtained supernatant; 2, carrying out 50 DEG C blow drying on the supernatant obtained in step 1 by a Termovap sample concentrator, adding a mobile phase solution to redissolve the dried supernatant, and allowing the redissolved supernatant to go through a 0.22 [mu]m micro-porous filtering film to obtain a sample to be detected; and 3, taking the sample to be detected, obtained in step 2, and qualitatively and quantitatively detecting the sample to be detected through LC-MS / MS. The marbofloxacin is used asthe internal standard substance, and the acetonitrile is used to precipitate the plasma proteins, so the extraction rate of the marbofloxacin from the dog blood plasma is greatly improved; and the marbofloxacin is quantitatively detected through the LC-MS / MS, so the method has the advantages of accurate detection result, simple steps in the detection process, high specificity and short time, and provides a fast and accurate analysis and determination method for clinical pharmacokinetics of the marbofloxacin.

Description

technical field

[0001] The invention relates to the field of detection methods, in particular to a detection method for drug residues. Background technique

[0002] Marbofloxacin is a fluoroquinolone antibacterial drug specially used for veterinary use. Marbofloxacin acts on broad-spectrum aerobic Gram-negative and some Gram-positive bacteria, as well as Mycoplasma, and has high bactericidal activity. As a third-generation marbofloxacin drug, it also mainly targets replication and transcription enzymes, such as DNA helicase and topoisomerase IV, both of which are important for bacterial survival.

[0003] Marbofloxacin can be well absorbed through oral administration and injection administration, and has low toxicity and side effects, so it is increasingly used in the treatment of deep and superficial skin infections and urinary tract infections in dogs.

[0004] Establishing a detection method for marbofloxacin in dog plasma can guide clinical rational drug use. At prese...

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