A kind of detection method of blood-nourishing brain preparation
A technology for nourishing blood and brain and detection methods, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of long analysis time, small proportion of ingredients, quantitative determination of paeoniflorin, etc., and achieve large quantity and polar coverage big effect
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Embodiment 1
[0183] 1. Preparation of reference solution
[0184] Take an appropriate amount of paeoniflorin reference substance, weigh it accurately, add 70% ethanol to make a solution with a concentration of 0.05 mg / ml, shake well, and obtain the paeoniflorin reference substance solution;
[0185] Take an appropriate amount of the orange-yellow cassiatin reference substance, accurately weigh it, add 70% ethanol to make a solution with a concentration of 5.0 μg / ml, shake well, and obtain the orange-yellow cassiatin reference substance solution.
[0186] 2. Preparation of the test solution Take about 0.4 g of the serum brain granule, accurately weigh it into a stoppered Erlenmeyer flask, add 15 ml of 70% ethanol precisely, and ultrasonically treat it for 15 minutes, let it cool and then centrifuge (3000 rpm ) for 5 minutes, accurately draw 5ml of the supernatant, pass through the treated DIKMA PLS solid-phase extraction column (500mg, first pre-wash with 10ml of acetonitrile, then pre-wash...
Embodiment 2
[0194] 1. Preparation of reference solution
[0195] Take an appropriate amount of paeoniflorin reference substance, weigh it accurately, add 70% ethanol to make a solution with a concentration of 0.01 mg / ml, shake well, and obtain the paeoniflorin reference substance solution;
[0196] Take an appropriate amount of the orange-yellow cassiatin reference substance, accurately weigh it, add 70% ethanol to make a solution with a concentration of 1.5 μg / ml, shake well, and obtain the orange-yellow cassiatin reference substance solution.
[0197] 2. Preparation of the test solution Take about 0.4g of the serum brain granule, accurately weigh it into a stoppered Erlenmeyer flask, add 10ml of 70% ethanol precisely, and ultrasonically treat it for 20 minutes, let it cool and then centrifuge (3000 rpm ) for 5 minutes, accurately draw 5ml of the supernatant, pass through the treated DIKMA PLS solid-phase extraction column (500mg, first pre-wash with 10ml of acetonitrile, then pre-wash w...
Embodiment 3
[0205] 1. Preparation of reference solution
[0206] Take an appropriate amount of paeoniflorin reference substance, weigh it accurately, add 70% ethanol to make a solution with a concentration of 0.10 mg / ml, shake well, and obtain the paeoniflorin reference substance solution;
[0207] Take an appropriate amount of the orange-yellow cassiatin reference substance, accurately weigh it, add 70% ethanol to make a solution with a concentration of 20 μg / ml, and shake well to obtain the orange-yellow cassiatin reference substance solution.
[0208] 2. Preparation of the test solution Take about 0.3 g of Yangxue Xuenao Pills, accurately weigh it into a stoppered Erlenmeyer flask, add 25 ml of 70% ethanol precisely, and process it with supersonication for 25 minutes, let it cool and then centrifuge (3000 rpm ) for 5 minutes, accurately draw 5ml of the supernatant, pass through the treated DIKMA PLS solid-phase extraction column (500mg, first pre-wash with 10ml of acetonitrile, then pr...
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