The present invention relates to a detection method of nourishing serum and brain preparation, said method is high-performance liquid chromatography, comprising the following steps: step 1, preparation of test solution, step 2, preparation of reference solution, step 3, chromatography Condition; Step 4, assay, need testing solution and reference substance solution are injected high-performance liquid chromatograph, obtain chromatogram. In the method provided by the present invention, after the test sample is processed by a solid-phase chromatographic column, the components are analyzed after enrichment and purification, so that the analysis time of the corresponding chromatographic method is shorter, and at the same time, the separation of chromatographic peaks and the weight of the method are guaranteed. and the present invention clearly defines the relative quantitative mode of the fingerprint, and clearly defines the control requirements of the absolute quantitative index (2) of the content determination and the relative quantitative index (19) of the fingerprint, and the control index is more comprehensive, to The quality attributes of the product are described more quantitatively.