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Method for determining content of harpagoside in radix scrophulariae medicinal material

A technology for the determination of harpagoside and its method, which is applied in the field of content determination of harpagoside in Scrophulariae Radix Scrophulariae, which can solve the problems of signal fluctuation and dependence on the production process level of instruments, achieve objective result standards, and improve the method and scope of the content determination of medicinal materials , the effect of satisfying the linear requirement

Pending Publication Date: 2022-07-29
张勇纯
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Problems solved by technology

[0005] Within a certain mass concentration range, the detection signal of the instrument and the injection amount correspond to each other and have a good linear relationship. This is a conventional and traditional quantitative determination method. This quantitative method based on the numerical signal on the detection instrument relies heavily on the production of the instrument itself. The level of craftsmanship has introduced many unstable factors, such as signal fluctuations

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  • Method for determining content of harpagoside in radix scrophulariae medicinal material
  • Method for determining content of harpagoside in radix scrophulariae medicinal material
  • Method for determining content of harpagoside in radix scrophulariae medicinal material

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Embodiment Construction

[0056] In order to make the technical solution of the present invention clearer and clearer to those skilled in the art, the present invention will be described in further detail below with reference to the embodiments and accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0057] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagents, materials, etc. used in the following examples can be obtained from commercial channels unless otherwise specified.

[0058] Control substance Harpagoside (Harpagoside, batch number: MUST-12071801, Chengdu Munster); chromatography grade acetonitrile, methanol (Fisher, USA); mass spectrometry grade formic acid (Clamar, China); Watsons distilled drinking water.

[0059] Eight batches of Scrophularia Radix were purchased from Hebei Anguo Medicinal Materials Market, Scrophularia GAP Base, Internet Medicinal Materials Plat...

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Abstract

The invention discloses a method for determining the content of harpagoside in a radix scrophulariae medicinal material, which belongs to the technical field of content determination methods, and comprises the following steps: preparing a standard solution, detecting by adopting an ultrahigh pressure liquid chromatography-tandem single quadrupole mass spectrometry detector, recording a mass spectrum, determining the peak intensity of main ions and adduct ions, calculating the ratio of the intensity, and determining the content of harpagoside in the radix scrophulariae medicinal material according to the content of harpagoside in the radix scrophulariae medicinal material. Drawing a standard curve of the ratio and the content; under the same condition, the test solution is detected, the content of harpagoside in the medicinal material is obtained according to the standard curve, and the method mainly adopts a quantitative method in which the ratio of the ion strength of the components on a single quadrupole mass spectrometry detector replaces a numerical detection signal of an instrument.

Description

technical field [0001] The invention relates to a content determination method, in particular to a content determination method for harpagoside in Scrophulariaceae medicinal materials, and belongs to the technical field of content determination methods. Background technique [0002] Scrophulariaceae is the dry root of Scrophulariaceae plant, sweet, bitter, salty, slightly cold, with functions of clearing heat and cooling blood, nourishing yin and reducing fire, detoxifying and dispersing knots. [0003] The main chemical constituents in Scrophularia are iridoids, phenylpropanoids, terpenoids, phenol and phenylethanoid glycosides, flavonoids and steroids. It has good pharmacological activity in diseases, treatment of diabetes, liver protection, anti-tumor, anti-oxidation, etc. Harpagoside is an iridoid glycoside component and is one of the main functional components of Scrophulariaceae. The content in the Chinese Pharmacopoeia is included in the 2020 edition of the Chinese P...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/60G01N30/72
CPCG01N30/02G01N30/60G01N30/72G01N2030/027G01N30/8631
Inventor 张勇纯黄建梅张洁
Owner 张勇纯
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