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Lipoprotein cholesterol detection method and kit

A kit and cholesterol technology, which is applied in biochemical equipment and methods, measuring devices, microbiological determination/inspection, etc., can solve the problems of large low-end deviation of determination reagents, inability to achieve accurate detection, large deviation of external quality assessment, etc. , to achieve high accuracy, meet the linear requirements of reagents, and good stability

Active Publication Date: 2022-03-22
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has been found through experiments that the low-end deviation of the reagents measured by this method is large, the linearity is poor, and the external quality assessment deviation is large, which cannot meet the requirements of accurate detection.

Method used

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  • Lipoprotein cholesterol detection method and kit
  • Lipoprotein cholesterol detection method and kit
  • Lipoprotein cholesterol detection method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The preparation of embodiment 1 kit

[0056] Taking the small and dense low-density lipoprotein cholesterol detection kit in the prior art as an example, the kit is divided into two reagent compositions, R1 and R2, and the specific formula is as follows:

[0057]

Embodiment 2

[0058] Example 2 Determination method

[0059] Mix the serum sample to be tested with reagent R1, keep the temperature at 37°C for 5 minutes, and measure the absorbance A 1 , then add reagent R2, mix well, keep the temperature at 37°C for 5min, measure the absorbance A 2 , calculate ΔA=A 2 -A 1

[0060] (2) Use a fully automatic biochemical analyzer (Hitachi 7180) to measure the absorbance difference after the reaction (the main wavelength is 546nm, the secondary wavelength is 660nm, and the reading point of the instrument is 16-34);

[0061] (3) Calculate the concentration of sdLDL-C in the sample according to the change value of absorbance.

[0062] Preferably, for the serum sample described in step (1), the volume ratio of the reagent R1 to the reagent R2 is 3:150:50.

[0063] (4) Linear experimental method:

[0064] Use high-concentration samples close to the upper limit of the linear range and low-concentration samples close to the lower limit of the linear range, a...

Embodiment 3

[0085] Example 3 Concentration Screening of Color Source Substances

[0086] 1. Experimental method:

[0087] According to the components and contents of the kit in Table 1, prepare reagents containing different color source substances, wherein TOOS is used as the color source material, and the concentrations are: 0.4mM, 0.8mM, 1.6mM, 2.0mM, 3.2mM. Use reagents with different TOOS concentrations for linearity verification, and the linearity method and requirements are the same as above.

[0088] 2. Experimental results:

[0089]

[0090]

[0091]

[0092] It is found through experiments that the correlation coefficient r of the assay kit meets the requirements, but from Table 2, it can be seen that the low-end deviation of the reagent kit in Table 1 is relatively large, and the linearity is poor. The present invention adjusts the concentration of the color source substance , when the concentration is 0.4mM-3.2mM, it does not conform to the range of 40-200mg / L, and t...

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Abstract

The invention discloses a lipoprotein cholesterol grading determination kit and a detection method suitable for an automatic analyzer, and a reagent used for the determination, the method and the reagent can be carried out without pretreatment of a sample, and the method and the reagent have the advantages of good specificity, rapidness and convenience in analysis. Meanwhile, the determination reagent can meet the linear standard of the reagent under the condition that the clinical detection result is not influenced, and has the characteristics of high accuracy and good stability.

Description

technical field [0001] The present invention relates to a method and a reagent for measuring lipoprotein cholesterol, in particular to a method and a reagent for detecting lipoprotein cholesterol related to the diagnosis of arteriosclerosis. Background technique [0002] Cholesterol is an important component of the cell and is clinically important because too high a level of cholesterol leads to the uptake of cholesterol by macrophages in the subendothelial space causing macrophages to transform into foam cells and This in turn leads to the development of primary lesions of atherosclerosis. Low-density lipoprotein (LDL) plays a major role in cholesterol transport in the blood and is a risk factor for arteriosclerosis. Small dense LDL, which is particularly small in size in LDL and denser than standard LDL, is known to have an atherogenic potential several times higher than normal LDL levels. The increase of small and dense LDL is one of the main risk factors of arterioscle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/28C12Q1/26
CPCC12Q1/60C12Q1/44C12Q1/28C12Q1/26C12Q2326/32G01N2333/908G01N2333/916G01N2333/918G01N2333/904G01N2800/044G01N2800/323
Inventor 李元丽芮海涛李强马腾飞
Owner ZYBIO INC
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