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Lipoprotein cholesterol detection method and kit

A technology of kits and reagents, applied in biochemical equipment and methods, measuring devices, microbiological determination/inspection, etc., can solve the problems of large low-end deviation of determination reagents, inability to achieve accurate detection, large deviation of external quality assessment, etc. , achieving high accuracy, meeting reagent linearity requirements, and good stability

Active Publication Date: 2022-07-12
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it has been found through experiments that the low-end deviation of the reagents measured by this method is large, the linearity is poor, and the external quality assessment deviation is large, which cannot meet the requirements of accurate detection.

Method used

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  • Lipoprotein cholesterol detection method and kit
  • Lipoprotein cholesterol detection method and kit
  • Lipoprotein cholesterol detection method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Preparation of the kit

[0056] Taking the small and dense low-density lipoprotein cholesterol detection kit in the prior art as an example, the kit is divided into two reagent compositions, R1 and R2, and the specific formula is as follows:

[0057]

Embodiment 2

[0058] Example 2 Measurement method

[0059] Mix the serum sample to be tested with reagent R1, keep the temperature at 37 °C for 5 min, and measure the absorbance A. 1 , then add reagent R2, mix well, keep constant temperature at 37°C for 5min, and measure the absorbance A 2 , calculate ΔA=A 2 -A 1

[0060] (2) Use an automatic biochemical analyzer (Hitachi 7180) to measure the difference in absorbance after the reaction (main wavelength 546nm, secondary wavelength 660nm, instrument reading point 16-34);

[0061] (3) Calculate the concentration of sdLDL-C in the sample according to the change in absorbance.

[0062] Preferably, in the serum sample described in step (1), the volume ratio of reagent R1 to reagent R2 is 3:150:50.

[0063] (4) Linear experimental method:

[0064] Mix the high concentration sample close to the upper limit of the linear range and the low concentration sample close to the lower limit of the linear range into 7 dilution concentrations (see the ...

Embodiment 3

[0085] Example 3 Color source substance concentration screening

[0086] 1. Experimental method:

[0087] According to the kit components and contents in Table 1, reagents containing different chromogenic substances were prepared, wherein the chromogenic substances were TOOS, and the concentrations were: 0.4 mM, 0.8 mM, 1.6 mM, 2.0 mM, 3.2 mM, respectively. Linearity verification was performed with reagents with different TOOS concentrations, and the linearity practices and requirements were the same as above.

[0088] 2. Experimental results:

[0089]

[0090]

[0091]

[0092] It is found through experiments that the correlation coefficient r of the assay kit complies with the regulations, but from Table 2, it can be seen that the reagents in the kit in Table 1 have large low-end deviation and poor linearity, and the present invention adjusts the concentration of color source substances. , at the concentration of 0.4mM-3.2mM, it does not meet the range of 40-200mg...

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Abstract

The invention discloses a kit and a detection method for graded determination of lipoprotein cholesterol suitable for an automatic analyzer, as well as a reagent for such determination. The method and reagent can be performed without pretreatment of samples, and have good specificity It also ensures that the assay reagent can meet the reagent linearity standard, high accuracy and good stability without affecting the clinical test results.

Description

technical field [0001] The present invention relates to methods and reagents for determining cholesterol in lipoproteins, in particular to methods and reagents for lipoprotein cholesterol detection related to the diagnosis of arteriosclerosis. Background technique [0002] Cholesterol is an important component of cells and is also clinically important because excessive levels of cholesterol cause macrophages in the subendothelial space to convert macrophages into foam cells after taking up cholesterol. This in turn leads to the development of primary lesions of arteriosclerosis. Low density lipoprotein (LDL) plays a major role in cholesterol transport in the blood and is a risk factor for arteriosclerosis. It is known that small, dense LDLs, which are particularly small in particle size among LDLs and are denser than standard LDLs, have atherogenic capacity at levels several times greater than normal LDLs. An increase in small, dense LDL is one of the major risk factors fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/28C12Q1/26
CPCC12Q1/60C12Q1/44C12Q1/28C12Q1/26C12Q2326/32G01N2333/908G01N2333/916G01N2333/918G01N2333/904G01N2800/044G01N2800/323
Inventor 李元丽芮海涛李强马腾飞
Owner ZYBIO INC
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