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High performance liquid chromatography measuring method for 10-HDA content in royal jelly soft capsule

A technology of high performance liquid chromatography and 10-HDA, which is applied in the field of high performance liquid chromatography for determining the content of 10-HDA in royal jelly soft capsules, can solve the problems of inability to accurately determine the content of 10-HDA in royal jelly soft capsules and the like, and achieves an accurate method. High sensitivity and sensitivity, resolving content instability, and good reproducibility

Inactive Publication Date: 2009-08-12
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to overcome the problem that cannot accurately measure 10-HDA content in the royal jelly soft capsule in the prior art, provide a kind of high-performance liquid chromatography determination method of 10-HDA content in the determination royal jelly soft capsule

Method used

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  • High performance liquid chromatography measuring method for 10-HDA content in royal jelly soft capsule
  • High performance liquid chromatography measuring method for 10-HDA content in royal jelly soft capsule
  • High performance liquid chromatography measuring method for 10-HDA content in royal jelly soft capsule

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Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Determination of Royal Jelly Soft Capsule 10-HDA Content

[0027] 1 material

[0028] 1.1 Instruments and materials

[0029] Dionex P680 series high performance liquid chromatograph, equipped with 4-channel UV detector; water bath or electric furnace; homogenizer; pH test paper (1-5).

[0030] 1.2 Drugs and reagents

[0031] Methanol (chromatographically pure), water is deionized water. Sodium hydroxide (analytical pure), hydrochloric acid (analytical pure), phosphoric acid (excellent pure), 10-HDA reference substance (Beijing Land Bridge Company), absolute ethanol (analytical pure), 10% sodium hydroxide solution.

[0032] The test sample is royal jelly soft capsules (100 capsules per bottle) produced by Zhongmi Company, Institute of Bee Research, Chinese Academy of Agricultural Sciences.

[0033] 1.3 Reference substance stock solution

[0034] Take by weighing 25mg of 10-HDA reference substance in 25mL capacity, dissolve with absolute ethanol, and consta...

experiment example 2

[0060] The test sample is the royal jelly soft capsule (sample 1) produced by Zhongmi Company of the Honey Bee Research Institute of the Chinese Academy of Agricultural Sciences, and the samples 2, 3 and 4 are three kinds of commercially available soft capsules.

[0061] Measure respectively the 10-HDA content of four kinds of soft capsule samples according to the method of embodiment 1, and the results are shown in the following table:

[0062] Table 1 Changes of 10-HDA content

[0063]

[0064] Experiments show that: according to the method of the present invention, the 10-HDA content of the royal jelly soft capsule stored within half a year (at a temperature of 4° C.) is measured, and the 10-HDA content does not change significantly.

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Abstract

The invention provides a high performance liquid chromatography method for detecting content of 10-HDA in a royal jelly soft capsule. An alkaline hydrolysis method is adopted for breaking the shell of the soft capsule; and after the pH value is regulated to be less than or equal to 5.0 by an acidity regulator (phosphoric acid, hydrochloric acid, sulfuric acid, formic acid or acetic acid), the anhydrous alcohol is used for ultrasonic extraction. The method has the advantages of effectively detecting the content of the 10-HDA in the royal jelly soft capsule, along with high accuracy and sensitivity and good repeatability.

Description

technical field [0001] The present invention relates to a high performance liquid chromatography method for determining the content of 10-hydroxyl-2-decenoic acid (abbreviated as 10-HDA) in royal jelly soft capsules, more specifically relates to a method for determining the content of royal jelly freeze-dried powder, salad High performance liquid chromatography method for 10-HDA content in royal jelly soft capsules made of oil, gelatin, glycerin, water, beeswax, lecithin and other materials. The technical key of the method is the processing method of soft capsule samples and the content of 10-HDA in whole capsules. Background technique [0002] Royal jelly is a special milky substance secreted by the nutrient glands (tongue and jaw glands) on the head of worker bees and used to feed the queen bee and larvae. It is milky white or light yellow, slightly sweet and sour. , spicy taste, fresh royal jelly is also called bee milk, royal jelly, royal jelly and so on. Due to the ta...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02
Inventor 薛晓锋田文礼吴黎明周金慧赵静陈兰珍
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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