Capillary electrophoretic method for measuring polymyxins antibacterial peptide and running buffer solution thereof
A technology of capillary electrophoresis and polymyxin, which is applied in the field of analytical chemistry, can solve the problems of low column efficiency and poor separation effect of polymyxin antimicrobial peptides, and achieve low cost, low consumption of samples and reagents, and easy The effect of using less reagent
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Embodiment 1
[0046] This example is a preferred running buffer for the determination of polymyxin antimicrobial peptides based on capillary electrophoresis in the present invention.
[0047] In this embodiment, the running buffer is a triethanolamine solution containing hydroxypropyl-β-cyclodextrin and isopropanol; wherein, in the buffer, the concentration of hydroxypropyl-β-cyclodextrin is 30 mmol / L, the content of isopropanol is 5% (volume fraction), the concentration of triethanolamine is 130mmol / L, and the pH is adjusted to 2.5 through phosphoric acid.
[0048] The experimental conditions of the capillary electrophoresis of the running buffer are: non-coated fused silica capillary, the column length is 60cm, the inner diameter is 50μm; the working voltage is 24kV; the column temperature is 25°C; the detection wavelength is 192nm; the injection pressure is 50mbar, and the injection time is 3s.
Embodiment 2
[0050] This example is a preferred running buffer for the determination of polymyxin antimicrobial peptides based on capillary electrophoresis in the present invention.
[0051] In this embodiment, the running buffer is a triethanolamine solution containing hydroxypropyl-β-cyclodextrin and isopropanol; wherein, in the buffer, the concentration of hydroxypropyl-β-cyclodextrin is 50 mmol / L, the content of isopropanol is 10% (volume fraction), the concentration of triethanolamine is 100mmol / L, and the pH is adjusted to 3 through sulfuric acid.
[0052] The experimental conditions of capillary electrophoresis for the running buffer are: cationic coated capillary, column length 100cm, inner diameter 100μm; working voltage 20kV; column temperature 30°C; detection wavelength 180nm; injection pressure 30mbar, injection time 5s.
Embodiment 3
[0054] This example is a preferred running buffer for the determination of polymyxin antimicrobial peptides based on capillary electrophoresis in the present invention.
[0055] In this embodiment, the running buffer is triethanolamine solution containing hydroxypropyl-α-cyclodextrin and acetonitrile; wherein, in the buffer, the concentration of hydroxypropyl-α-cyclodextrin is 5mmol / L , the content of acetonitrile is 8% (volume fraction), the concentration of triethanolamine is 80mmol / L, and the pH is adjusted to 2 by hydrochloric acid.
[0056] The experimental conditions of capillary electrophoresis for the running buffer are: anion-coated capillary, column length 30cm, inner diameter 25μm; working voltage 10kV; column temperature 40°C; detection wavelength 380nm; injection pressure 40mbar, injection time 10s.
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