Method for constructing Compound Xueshuantong preparation HPLC fingerprint pattern

The technology of compound Xueshuantong and fingerprint spectrum is applied in the field of construction of HPLC fingerprint of compound Xueshuantong preparations, which can solve the problems of lack of detection of chemical components of four medicinal materials, inability to monitor the stability of production process, and inability to comprehensively monitor intermediates, etc. Good specificity, good reproducibility, easy to generalize effect

Active Publication Date: 2010-04-21
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fufang Xueshuantong Capsules are produced using 50% ethanol extraction process, so the components in the finished product include both water-soluble components and fat-soluble components, the components are complex, and the polarities are greatly different. The TLC spots of Danshen reference medicinal material and protocatechualdehyde reference substance under each specific chromatographic condition are for contrast identification, and TLC scanning is used to determine the total amount of ginsenoside Rg1 and Rb1. The disadvantages are:
[0003] 1. There is no comprehensive and systematic detection of the chemical components in the four herbs
[0004] 2. No detection of Scrophulariaceae
[0005] 3. The quality of intermediates and finished products cannot be fully monitored
[0006] 4. The stability of the production process cannot be monitored

Method used

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  • Method for constructing Compound Xueshuantong preparation HPLC fingerprint pattern
  • Method for constructing Compound Xueshuantong preparation HPLC fingerprint pattern
  • Method for constructing Compound Xueshuantong preparation HPLC fingerprint pattern

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Construction method of HPLC fingerprint of compound Xueshuantong capsule and HPLC fingerprint of compound Xueshuantong capsule

[0062] 1. Instruments and reagents

[0063] 1.1 Instrument

[0064] Electronic analytical balance; ultrasonic cleaner; rotary evaporator; ultrapure water device; ODS solid phase extraction column; liquid chromatography; high performance liquid chromatography-mass spectrometry system chromatography; ultraviolet detector; mass spectrometer detector.

[0065] Column:

[0066] ①Merck Lichrospher Rp-18e: 5μm, 250×4.0mm;

[0067] ②Agilent Hypersil: 5μm, 250×4.0mm;

[0068] ③Elite Lichrosorb Rp-18e: 5μm, 250×4.6mm;

[0069] ④Dima Diamonsil C18: 5μm, 250×4.6mm;

[0070] ⑤Dalian Elite Hypersil C18: 5μm, 250×4.0mm

[0071] 1.2 Reagent

[0072] Reference substance

[0073]

[0074] Control herbs

[0075]

[0076] 2. Methods and Results

[0077] 2.1 Chromatographic conditions

[0078] Chromatographic column: a chromatographic c...

Embodiment 2

[0133] Embodiment 2: the construction method of Scrophulariaceae HPLC fingerprint

[0134] 1. Instruments and reagents

[0135] 1.1 Instruments

[0136] Electronic analytical balance; ultrasonic cleaner; rotary evaporator; ultrapure water; ODS solid phase extraction cartridge; liquid chromatograph; chromatograph of high performance liquid chromatography-mass spectrometry system;

[0137] Column:

[0138] ①Merck Lichrospher Rp-18e: 5μm, 250×4.0mm;

[0139] ②Agilent Hypersil: 5μm, 250×4.0mm;

[0140] ③Elite Lichrosorb Rp-18e: 5μm, 250×4.6mm;

[0141] ④Dima Diamonsil C18: 5μm, 250×4.6mm;

[0142] ⑤Dalian Elite Hypersil C18: 5μm, 250×4.0mm

[0143] 1.2 Reagents

[0144] The samples are all medicinal materials put into production by Guangdong Zhongsheng Pharmaceutical Co., Ltd., and they are the varieties specified in the Pharmacopoeia. They are tested according to the medicinal materials standards recorded in the Chinese Pharmacopoeia, and they are all qualified medicinal m...

Embodiment 3

[0171] Embodiment 3: the construction method of Panax notoginseng HPLC fingerprint

[0172] 1. Instruments and reagents

[0173] 1.1 Instruments

[0174] Electronic analytical balance; ultrasonic cleaner; rotary evaporator; ultrapure water; ODS solid phase extraction cartridge; liquid chromatograph; chromatograph of high performance liquid chromatography-mass spectrometry system;

[0175] Column:

[0176] ①Merck Lichrospher Rp-18e: 5μm, 250×4.0mm;

[0177] ②Agilent Hypersil: 5μm, 250×4.0mm;

[0178] ③Elite Lichrosorb Rp-18e: 5μm, 250×4.6mm;

[0179] ④Dima Diamonsil C18: 5μm, 250×4.6mm;

[0180] ⑤Dalian Elite Hypersil C18: 5μm, 250×4.0mm

[0181] 1.2 Reagents

[0182]The samples are all medicinal materials put into production by Guangdong Zhongsheng Pharmaceutical Co., Ltd., and they are the varieties specified in the Pharmacopoeia. They are tested according to the medicinal materials standards recorded in the Chinese Pharmacopoeia, and they are all qualified medicinal m...

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Abstract

This invention relates to compound thrombus-relaxing agent fingerprint spectrum recreation method by use of natural plants medicinal materials, which comprises the following parts: test solution process; comparing liquid process; measuring with effective liquid chromatograph measurement with chromatograph relative keeping time and peak area as one; computing the test items relative keeping time and relative peak area to get the HPLC fingerprint chromatograph. The required HPLC standard fingerprint chromatograph to notoginseng chromatograph has five common peaks; the one to astragalus root hastwo common peaks; chromatograph to radix salvia miltiorrhiza has two common peaks; one to scrophularia root has two common peaks; the one to radix salvia miltiorrhiza under 270 nm has three common peaks; the one to scrophularia root has common peak.

Description

technical field [0001] The invention relates to a method for constructing an HPLC fingerprint of a compound Xueshuantong preparation prepared from natural plant medicinal materials, and the HPLC standard fingerprint of a compound Xueshuantong preparation obtained by the method. Background technique [0002] The fingerprint of traditional Chinese medicine refers to the chromatographic or spectral spectrum of a certain type of composition that is common to some or several kinds of Chinese medicinal materials. At present, the quality control technology of traditional Chinese medicine fingerprints, as a detection method to control the quality uniformity and stability of traditional Chinese medicines, is one of the key technologies in the modernization of traditional Chinese medicines, which is of great significance to effectively control the quality of traditional Chinese medicinal materials. Chromatographic fingerprint quality control technology is the highlight of the current ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/02G01N33/06G01N33/60
Inventor 苏薇薇龙超峰白杨谢称石彭维杜静王永刚
Owner SUN YAT SEN UNIV
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