Enrichment and characterization method of American ginseng polypeptide
A technology of American ginseng and enrichment, which is applied in the fields of separation analysis, plant omics and biology, can solve the problems such as poor knowledge of American ginseng peptides, and achieve high throughput and sensitivity, high sensitivity, and fast analysis speed
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Embodiment 1
[0045] 1. Enrichment and separation of target peptides
[0046] Crush and dry American ginseng slices 100g, add a volume ratio of 50% ethanol aqueous solution as the extraction solvent, the mass volume ratio of the medicinal material powder to the solvent is 0.125g / ml, mix well; ultrasonic for 30 minutes, separate, collect the solvent, repeat the extraction twice, and combine Extract solution; dry the resulting solution under a gentle nitrogen flow, and then redissolve it in 100mL water; add the extract to 300ml ethanol for alcohol precipitation, and dissolve the precipitate in 40ml water; add it to the suspension at a volume ratio of 1:1 Add 40ml of ethyl acetate, repeat 3 times to obtain the extract containing ethyl acetate and the extract not containing ethyl acetate; take the extract not containing ethyl acetate, add 40ml n-butanol according to the volume ratio of 1:1, Repeat 3 times to obtain the extract containing n-butanol and the extract of the aqueous layer; use the A...
Embodiment 2
[0059] 1. Enrichment and separation of target peptides
[0060] Crush and dry American ginseng slices 100g, add a volume ratio of 30% ethanol aqueous solution as the extraction solvent, the mass volume ratio of the medicinal material powder to the solvent is 0.2g / ml, mix evenly; ultrasonic for 30 minutes, separate, collect the solvent, repeat the extraction twice, and combine Extract solution; dry the resulting solution under a gentle nitrogen flow, and then redissolve it in 200mL water; add the extract to 400ml ethanol for alcohol precipitation, and dissolve the precipitate in 50ml water; add to the suspension at a volume ratio of 1:1 Add 50ml of ethyl acetate, repeat 3 times to obtain the extract containing ethyl acetate and the extract not containing ethyl acetate; take the extract not containing ethyl acetate, add 50ml n-butanol according to volume ratio 1:1, Repeat 3 times to obtain the extract containing n-butanol and the extract of the aqueous layer; use the Amicon Ultr...
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