Method for disinfecting surface of embryo egg for producing vaccine

Abstract

The invention relates to a method for disinfecting the surface of an embryo egg for producing a vaccine. The method comprises the following steps: (1) treating an aqueous hydrogen peroxide solution into a dry fog state, and treating the embryo egg for 40-60 minutes; and (2) spraying the embryo egg with a 75% ethanol solution, and performing secondary disinfection treatment. The vaccine is an antiviral vaccine, and the virus can be cultured by the embryo egg.

Description

Technical field [0001] The present invention belongs to the field of medical technology, and in particular, a method involving a method of disinfecting the surface of the embryo eggs for the production of vaccines. Background technique [0002] Influenza is a large-scale infectious disease that is unable to control so far. It will cause acute upper respiratory infections, and rapidly spread through air, there is often a periodic popularity in the world, and the incidence of influenza has been high, and the compass caused by the compass is very serious, leading to mortality Higher. According to data released by the World Health Organization (WHO), the world's annual influenza is 600 million to 1.5 billion, death 25-500,000, which brings serious harm to human health. In order to control and prevent influenza, WHO encourages action to improve the awareness of medical personnel and public to inoculate flu vaccination, including the target of national immunization plan. [0003] The i...

AI Technical Summary

Problems solved by technology

Formaldehyde + potassium permanganate has a good bactericidal effect on the microorganisms on the surface of eggs, but formaldehyde is a harmful gas that can coagulate protein, has a strong stimulating effect on the skin, and is toxic and damaging to human organs. Long-term exposure to formaldehyde can cause breathing Functional dysfunction and liver poisoning, etc., should be fully ventilated after fumigation, which is time-consuming and laborious, and the residual toxicity is not easy to remove. Therefore, it is not suitable to use formaldehyde + potassium permanganate fumigation to control surface microorganisms before virus inoculation. At present, alcohol is mostly used. Spray a disinfection method to control surface microorganisms. The principle of disinfection is to use the strong water absorption of alcohol to dehydrate, denature and precipitate the protein of the bacteria. Since the protein of the bacteria is one of the main components of the bacteria itself, it can be destroyed when it is destroyed. To achieve the purpose of sterilization

75% alcohol is a medium-efficiency disinfectant, which can kill Mycobacterium tuberculosis, but cannot kill microorganisms such as bacterial spores, some fungi, viruses, etc. Microorganisms that have not been killed may continue to reproduce or release toxins, and it takes a certain time to act. Play a killing role, and alcohol is very volatile. After spraying on the surface of embryonated eggs, as the concentration decreases, the disinfection ability gradually weakens. Only a single disinfection method is used before inoculation of embryonated egg virus—75% alcohol spray disinfection cannot completely reduce Surface Microbial Control Risks

However, when it is used to disinfect the surface of embryonated eggs, too high concentration of hydrogen peroxide or too long action time may damage the embryonated eggs and affect the viability of embryos. Damaged embryonated eggs are not conducive to the proliferation of viruses and affect the yield of viruses. , it is necessary to find the appropriate hydrogen peroxide concentration and time

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