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Application of ghmyb36 gene in improving plant resistance to Verticillium wilt

A Verticillium wilt, R2R3MYB technology, applied in the fields of application, plant peptides, plant products, etc., can solve the problem of less research on cotton resistance to Verticillium wilt, achieve the effect of reducing the average incidence rate and enriching resistance gene resources

Active Publication Date: 2021-04-27
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

R2R3 type MYB proteins mainly play an important role in plant growth and development, metabolic regulation and drought tolerance Cedroni et al., 2003; Wang et al., 2004; Wu et al.,2006; Pu et al., 2008; Machado et al. al., 2009; Guan et al., 2014), and less research on cotton resistance to Verticillium wilt

Method used

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  • Application of ghmyb36 gene in improving plant resistance to Verticillium wilt
  • Application of ghmyb36 gene in improving plant resistance to Verticillium wilt
  • Application of ghmyb36 gene in improving plant resistance to Verticillium wilt

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Embodiment Construction

[0029] The methods used in the following embodiments are conventional methods unless otherwise specified, and the primer sequences used are all synthesized by Shanghai Handsome Biotechnology Co., Ltd., and the stated percentages are all mass percentages. The gene in this experiment was derived from upland cotton Ao 3503 ( Gossipium hirsutum ).

[0030] 1) Cotton GhMYB36 Induced expression analysis of

[0031] The cotton seedlings used were 2 weeks old, and the pathogenic bacteria induction conditions were as follows: the strain was deciduous type strong pathogenic bacteria V991. After the pathogenic bacteria are activated on the PDA plate, pick the bacterial block from the edge of the colony and put it into Chase culture medium (g / l): NaNO 3 2g, K2HPO 4 1g, MgSO 4 -7H 2 O 0.5g, KCl 0.5g, FeSO 4 -7H 2 O 0.01g, sucrose 30g, culture at 25°C, 180 r min for 5-6 days, filter the culture solution with gauze, examine under the microscope and count with a hemocytometer. The...

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Abstract

The present invention relates to methods for conferring resistance to Verticillium wilt in plants GhMYB36 The gene and its application belong to the field of biotechnology. GhMYB36 The gene is a R2R3 type MYB protein gene obtained from upland cotton Ao 3503 variety, which contains two DNA binding domains. GhMYB36 The Verticillium wilt disease resistance of the transgenic expression strain is significantly enhanced, not only can delay the onset, but also greatly reduce the average incidence rate. After the inoculation of pathogenic bacteria, the resistance of the transgenic line to Verticillium dahliae V991 was significantly enhanced.

Description

technical field [0001] The present invention relates to a plant that confers Verticillium wilt resistance GhMYB36 Genes and their application in plant resistance to Verticillium wilt. Background technique [0002] Cotton verticillium wilt is a soil-borne disease, which can cause a large number of buds and bolls to fall off. When the disease is serious, they will fall off into bare stems or even die, reducing cotton production by 20-60%. It is the most important disease in cotton production in China. So far, there is no effective control agent, and we can only rely on comprehensive control measures based on planting resistant varieties (Jian Guiliang et al., 2003; Zhang Baolong et al., 2012). However, most of the new cotton varieties promoted in my country in recent years are susceptible or resistant, and the lack of resistant varieties has made the damage of the disease increasingly serious (Jian Guiliang et al., 2003). Verticillium wilt resistance resources mainly exist i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/20
CPCC07K14/415C12N15/8282
Inventor 刘廷利阚家亮杨郁文张保龙陈天子凌溪铁
Owner JIANGSU ACAD OF AGRI SCI
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