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Method for identifying salt tolerance of peanuts in seedling stage

An identification method and technology of salt tolerance, applied in the field of salt tolerance identification in peanut seedling stage, can solve the problems of peanut root growth observation limitation, root damage, etc., and achieve the effect of ensuring consistency, maintaining integrity, and avoiding damage to the root system.

Inactive Publication Date: 2018-05-18
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods have limitations on the observation of peanut root growth, and may cause root damage during the sampling process

Method used

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  • Method for identifying salt tolerance of peanuts in seedling stage
  • Method for identifying salt tolerance of peanuts in seedling stage
  • Method for identifying salt tolerance of peanuts in seedling stage

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Embodiment 1

[0026] A method for identifying salt tolerance in peanut seedling stage, comprising the following steps:

[0027] S1: Material preparation

[0028] S11: The pods of newly harvested peanut varieties are air-dried and placed at room temperature, and the germination rate of seeds of each peanut variety is detected through a germination test, and the peanut variety with a germination rate higher than 90% is selected, and the pods of the peanut variety are shelled, and plump, Seeds of the same size are placed in a petri dish covered with two layers of filter paper, distilled water is added to keep the seeds in a suspended state, and the lid of the petri dish is covered;

[0029] S12: Soak the petri dish in water at a temperature of 28-30°C for 6-8 hours, pour off the water, add distilled water, and continue culturing for 60-72 hours;

[0030] S13: When most of the seeds germinate, put the seeds whose radicle length is greater than 1 cm in a germination bag, place 1 seed in each ge...

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Abstract

The invention relates to a method for identifying salt tolerance of peanuts in a seedling stage, and belongs to the technical field of agricultural field variety evaluation. The method comprises the steps of material preparation-salt stress treatment. According to the method, a novel method for identifying salt tolerance of the peanuts in the seedling stage is established, germination bags are used and Hoagland's nutrient solution is adopted for culturing so that dynamic changes of peanut root growth in a salt stress process can be observed to provide a basis for detection dynamic indicators of salt stress of the peanuts in the seedling stage, and meanwhile, integrity of a root system can be maintained to avoid damage to the root system when materials are drawn.

Description

technical field [0001] The invention belongs to the technical field of variety evaluation in the agricultural field, and in particular relates to a method for identifying salt tolerance of peanut seedlings. Background technique [0002] Peanut is an important oil crop and economic crop in my country. The growth process of peanut mainly includes the seed germination and emergence stage, the seedling stage, the flowering and needle stage, the pod setting stage and the pod maturity stage, etc. Among them, the seedling stage is one of the sensitive periods of peanut adversity stress, such as salt stress. The research at the seedling stage mainly adopts potting soil condition culture, nutrient solution culture method with vermiculite or quartz sand as the medium. Wang Baozhi (2010) carried out the method of cultivating seedlings when carrying out "cloning and expression profile analysis of peanut salt-tolerant genes": fresh peanut Linhua No. 5 was cultivated in vermiculite, wate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G31/00
Inventor 陈静邱俊兰胡晓辉苗华荣储烨张胜忠崔凤高杨伟强
Owner SHANDONG PEANUT RES INST
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