Seal cover used for biological reaction

A technology of biological reaction and sealing cover, which is applied in the preparation of test samples, sampling, and measuring devices. It can solve the problems of uneven heating, air bubbles and cavities, and long time consumption, so as to ensure heat uniformity and reduce heat loss. , The effect of saving the reaction solution

Pending Publication Date: 2018-06-08
杭州依美洛克医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process is time-consuming and requires manual monitoring, so there are coverslips that can be applied to tissue processing by automated means
This type of cover slip can be applied to automatic implementation. Both the front end and the back end of the cover slip are connected with the outside world, so that the reaction liquid can flow into the reaction area from the front end of the cover slip and flow out from the rear end of the reaction area, realizing liquid addition, cleaning and The automatic completion of liquid drainage, etc., but this kind of cover slip has the problem of air bubbles and cavities during thermal repair. This is because the thermal repair process is long and the temperature needs to reach 100 ° C, which exists between the slide and the cover slip Bubbles and evaporation will occur in the reaction liquid, and the addition of new reaction liquid during the heat repair process will affect the overall temperature of the heat repair. When heating, heat will be lost from both ends, resulting in heat loss and uneven heating

Method used

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  • Seal cover used for biological reaction
  • Seal cover used for biological reaction
  • Seal cover used for biological reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Such as Figure 10 As shown, the front part of this embodiment is a liquid addition area 12 for adding or storing the reaction solution, and the rear part is a reaction area 11 for forming a reaction chamber. The liquid addition area 12 and the reaction area 11 are connected by a drainage slope 13 , so that the reaction liquid in the liquid addition area 12 can smoothly enter the reaction area 11, of course, the drainage device is not limited to this kind of drainage slope 13, such as a reaction area with a guided side wall or top wall, and a different opening height. Different device forms such as opening the mouth or pressing the liquid into the reaction zone through the height difference can all achieve the desired effect of the drainage device. combined reference figure 2 , the reaction zone 11 is limited by the reaction zone top wall 111, the reaction zone side wall 112 and the reaction zone rear wall 113, and is enclosed with the slide 2 to form a semi-sealed re...

Embodiment 2

[0042] This embodiment is basically the same as Embodiment 1, the difference is that a through hole (not shown in the figure) is provided on the surface of the uppermost end of the sealing cover, and the through hole communicates with the notch 114, so that the sealing cover can be removed from the sealing cover during thermal repair. Air bubbles are simultaneously discharged at the front and rear ends of the unit without affecting thermal cycling.

Embodiment 3

[0044] Such as Figures 1 to 4 As shown, the front part of this embodiment is a liquid addition area 12 for adding or storing the reaction solution, and the rear part is a reaction area 11 for forming a reaction chamber. The liquid addition area 12 and the reaction area 11 are connected by a drainage slope 13 , the reaction zone 11 is limited by the reaction zone top wall 111, the reaction zone side wall 112 and the reaction zone rear wall 113, with reference to Figure 8 , the reaction zone 11 and the slide 2 are enclosed to form a semi-sealed reaction chamber, which at least includes the seal between the side wall 112 of the reaction zone and the rear wall 113 of the reaction zone and the slide 2, and the height of the reaction chamber is set to 0.1 mm ~ 0.15mm, the volume is 100 microliters ~ 120 microliters (not including the notch). The reaction zone top wall 111 is provided with an upward notch 114 near the reaction zone rear wall 113 position, and the volume of the not...

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Abstract

The invention relates to a seal cover used for biological reaction. The seal cover comprises a reaction zone and a liquid adding zone; the reaction zone comprises a reaction zone top wall, a reactionzone side wall, and a reaction zone back wall; the reaction zone top is provided with a recess close to the reaction zone back wall; the front end of the reaction zone is connected with the liquid adding zone through a flow guide device; in reaction, a reaction chamber is formed via matching of the reaction zone top wall, the reaction zone side wall, the reaction zone back wall, and a slide; the reaction zone side wall, the reaction zone back wall, and the slide are designed to match each other in a sealed manner; and a liquid enrichment zone is formed at the recess. The seal cover is capableof reducing influences of reaction liquid evaporation and bubble generation on experiment results in thermal remediation process; the seal cover is helpful in spreading of reaction liquid, and entering of reaction liquid into the reaction zone smoothly and rapidly; and the seal cover is helpful in circulation of heat energy in reaction liquid, and heat energy uniformity is ensured.

Description

technical field [0001] The invention relates to a biological experiment device, in particular to a sealing cover for biological reactions. Background technique [0002] In biological experiments, the samples are pre-treated, and then undergo a series of staining and other treatments, so that the experimenter can clearly observe the conditions of the samples. The usual treatment includes a series of steps such as pretreatment and staining. Normally, manual operation is used to place the sample tissue on the slide, and go through steps such as dewaxing, repairing, staining and dehydration. The above steps need to remove the coverslip from the slide. Take it off the top of the slide to add liquid, and then put the cover slip back on. This process is time-consuming and requires manual monitoring, so there are coverslips that can be applied to tissue processing by automated means. This type of cover slip can be applied to automatic implementation. Both the front end and the bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/2813
Inventor 陈鑫王成成李思勇余向东
Owner 杭州依美洛克医学科技有限公司
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