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Corn SSR (Solid-State Relay) marked rapid silver staining detection method for breeding improvement

A detection method, corn technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of long process time and limited reaction time.

Inactive Publication Date: 2018-06-15
ANHUI TIANQIN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the existing corn breeding industry, with the application of molecular breeding, the yield of corn is increasing. The existing corn marking process takes a long time and has restrictions on the reaction time

Method used

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Embodiment Construction

[0028] The technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0029] The present invention is a rapid silver staining detection method for corn SSR markers improved by breeding, including corn seeds: the corn seeds are provided by 28 different breeding units; SSR simple sequence repeat markers (Simple sequence repeat, referred to as SSR markers), also known as Microsatellite sequence repeats are a kind of repeated sequences consisting of several nucleotides (1-5) as repeating units, up to dozens of nucleotides, short in length, and widely distributed on chromosomes;

[0030] A kind of breeding improved corn SSR ...

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Abstract

The invention discloses a corn SSR (Solid-State Relay) marked rapid silver staining detection method for breeding improvement and relates to the technical field of corn breeding. Corn seeds are adopted, and the corn seeds are provided by 28 different breeding institutes. By using a rapid silver staining method, two steps of fixation and dyeing are integrated into one, developing can be carried outdirectly without dilution after dyeing, a clear banding pattern can be obtained within 2 minutes generally, the whole process is not longer than 10 minutes, in addition, the dyeing background is deepened for a certain extent along with time, and the reading results are not affected by long-term development.

Description

technical field [0001] The invention belongs to the technical field of corn breeding, in particular to a rapid silver staining detection method of corn SSR markers improved in breeding. Background technique [0002] Molecular breeding is a breeding method that applies genetic engineering to breeding work, through gene introduction, to breed new varieties with certain requirements. Animal molecular breeding methods are mainly based on molecular markers for marker-assisted selection, and then transgenic breeding based on transgenic technology. According to people's wishes, rigorous design is carried out, and through in vitro DNA recombination technology and DNA transfer technology, the biological species is purposefully transformed, so that existing species tend to be perfected in a short period of time to create new biological types. system, that is, genetic engineering. [0003] In the existing corn breeding industry, with the application of molecular breeding, the yield o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858
CPCC12Q1/6858C12Q2531/113C12Q2565/125C12Q2563/173
Inventor 林曙东
Owner ANHUI TIANQIN AGRI TECH CO LTD
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