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Preparation method of bone marrow chromosome G band

A production method and chromosome technology, applied in the field of biomedical testing, can solve the problems of lowering the success rate of chromosome karyotype analysis, small analyzable split images, lowering clinical practicability, etc., and achieving reliable diagnosis results, good dispersion, and lower production costs. cost effect

Inactive Publication Date: 2018-06-15
济南金域医学检验中心有限公司
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Problems solved by technology

Due to the complex process of making bone marrow chromosomes and many influencing factors, there are often phenomena such as small analyzable mitotic figures, thick and short chromosomes, and poor dispersion, which greatly reduces the success rate of karyotype analysis and its clinical practicability.

Method used

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  • Preparation method of bone marrow chromosome G band
  • Preparation method of bone marrow chromosome G band
  • Preparation method of bone marrow chromosome G band

Examples

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Embodiment 1

[0051] Example 1: Production of Bone Marrow Chromosome G Band

[0052] The specific production method includes the following steps:

[0053] (1) Prepare medium: take out RPMI1640 medium from the freezer, incubate in a 37°C water bath, and set aside.

[0054] (2) Inoculation: First, count the bone marrow cells: first destroy the red blood cells with the white blood cell diluent, and use the blood cell technology plate to count the cells in the four-corner large square to obtain the average number N, and determine the amount of bone marrow blood in the medium according to the average number N ;

[0055] When N≤20, the inoculum volume of bone marrow blood in every 5ml of bone marrow cell culture medium is 2.5ml;

[0056] When 20<N≤200, the inoculum volume of bone marrow blood in every 5ml of bone marrow cell culture medium is 50 / N ml;

[0057] When N>200, dilute the bone marrow blood to N'=80-120, then inoculate according to the inoculum amount of bone marrow blood in every 5m...

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Abstract

The invention discloses a preparation method of a bone marrow chromosome G band. The method comprises the following steps: (1) performing bone marrow white cell counting, determining the inoculation amount and culture time of bone marrow blood according to the result of the white blood cell counting; (2) inoculating the bone marrow blood into a bone marrow cell culture medium for culturing; (3) adding colchicine to the bone marrow cell culture medium to stop the culturing; (4) collecting a bone marrow cell culture, preparing the bone marrow cell culture into cell suspension, and adjusting theturbidity of the cell suspension to 2-3 McFarland turbidity; (5) dropping the cell suspension on a glass slide, burning the slide glass for 10 to 15 seconds, and after burning of the glass slide, baking the slide glass; and (6) treating the baked glass slide, and dyeing the glass slide with a Giemsa dye to obtain a G-banding chromosome sample. A bone marrow chromosome prepared by the method is inthe form of a long rod, and is well dispersed without crossover, and banding is clearly visible.

Description

technical field [0001] The invention relates to the technical field of biomedical testing, in particular to a method for making bone marrow chromosome G-band. Background technique [0002] With the development of molecular biology and cytogenetics, bone marrow staining karyotype analysis has played an increasingly important role in the diagnosis, treatment and prognosis of blood system diseases. During the study of chromosomes, researchers expect to obtain more information with identifying features, the so-called "anatomical" features of chromosomes, through experimental techniques that lead to differentiated staining of the internal structures of chromosomes. After different methods and different dyes are used to treat the chromosome specimens, the bands of light and dark or different shades appear on each chromosome. This differentiation and dyeing technology is the chromosome banding technology. G-banding is a widely used banding type at present. It is mainly developed a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/2813G01N1/30
Inventor 谢风祥王婷婷吴晓飞侯生根王丙维彭德志韦杰
Owner 济南金域医学检验中心有限公司
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