Manufacturing method for high-resolution peripheral blood G band

Abstract

The invention relates to a manufacturing method of a high-resolution peripheral blood G band. The method comprises that (1) leucocytes of peripheral blood are counted, and the inoculation amount and cultivation time of peripheral blood are determined according to a leucocyte counting result; (2) the peripheral blood is inoculated in a peripheral blood cell culture medium according to the inoculation amount in the step (1) and cultured for the cultivation time in the step (1); (3) 40ug / ml colchicine is added into every 5m peripheral blood cell culture medium, and culturing is ended; (4) a peripheral blood cell suspension is taken, and the concentration of the cell suspension is adjusted to 1.2-3.6*109 / L; (5) the cell suspension is dropped on a glass slide, and the glass slide is burned for9-16s; (6) the glass slide is baked after burning, and trypsin in the concentration of 0.025% is used to divide the baked slide glass into zones for 10-25s; and (7) dyeing is carried out for 5min by means of a Giemsa dye, and a chromosome specimen with a G band is obtained. The peripheral blood chromosome manufactured by the method is long-rod shaped, scattered well without intersection, the G band is clear, and the zone line level is high.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a method for making high-resolution peripheral blood chromosome G bands. Background technique [0002] China is a country with a high incidence of birth defects. According to the "Report on Prevention and Control of Birth Defects in China (2012)", at present, there are about 16 million newborns in my country every year, and the incidence of birth defects is about 5.6%. trend. Among them, about 35% of the children died after birth, and 40% of the children were permanently disabled. Not only were they plagued by birth defects for life, but they also brought heavy mental and economic burdens to families and society. According to the "Maternal and Child Health Development Report (2011)", since the founding of New China, the infant mortality rate in my country has continued to decline, but the birth defect rate has increased by nearly 70% in the past 15 years, from 97.7 / milli...

AI Technical Summary

Problems solved by technology

Due to the complex process of making peripheral blood chromosomes and many influencing factors, there are often phenomena such as small analyzable mitotic figures, thick and short chromosomes, and poor dispersion, which greatly reduces the success rate of chromosome karyotype analysis and reduces its clinical practicability. Routine chromosome analysis is difficult to detect abnormalities < 10M bases

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