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Tissue culture method for Hudieying

A tissue culture and culture method technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of restricting the application of cherry blossoms, low survival rate, proliferation coefficient rooting rate of less than 50%, etc., to achieve suitable promotion and rooting. The effect of good rate and good market prospect

Active Publication Date: 2018-07-13
SUQIAN AGRI SCI RES INST JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

In the tissue culture and rapid propagation process of cherry blossoms in the prior art: the germination rate of explants is about 30-40%, the multiplication coefficient is 1-2.4, and the rooting rate is less than 50%. The lower survival rate seriously restricts the use of cherry blossoms in urban greening and gardens. Applications under construction

Method used

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  • Tissue culture method for Hudieying
  • Tissue culture method for Hudieying
  • Tissue culture method for Hudieying

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Embodiment

[0022] A tissue culture method of Butterfly Cherry, said culture method comprises the following steps: (1) selection of explants: selected from the middle and upper part of the twigs of Butterfly Cherry that were born in the same year, strong and free from diseases and insect pests as explants, and the remaining leaves were removed. The petiole, the butterfly cherry is collected from the cherry blossom garden of Yunhewan Academy of Agricultural Sciences, Suyu District; (2) pretreatment of explants: use a hand-washing brush dipped in washing powder to gently scrub the surface of the stem section and the axillary buds, rinse with tap water, and then Cut into small sections of 2-3cm, each section has at least one lateral bud, rinse once through tap water; (3) disinfection of explants: after plant material is pretreated, proceed to ultra-clean workbench and carry out following disinfection treatment: 70% Soak in ethanol for 10s, then soak with 0.1% mercuric chloride plus 2 drops of...

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Abstract

The invention provides a tissue culture method for Hudieying. The tissue culture method comprises the following steps: disinfection of an explant; induction culture; proliferation culture; rooting culture; and seedling hardening and transplanting. The invention provides the tissue culture method for Hudieying to overcome defects in the prior art and allows tissue-cultured Hudieying to have high germination rate, great proliferation coefficient, good rooting rate, short culture period, high survival rate, good market prospects and promotion value.

Description

technical field [0001] The invention relates to the technical field of plant culture, in particular to a method for tissue culture of Butterfly Cherry. Background technique [0002] Cherry blossoms generally refer to the flowers of the genus Prunus in the Rosaceae Li subfamily. Cherry blossoms are woody plants. The traditional propagation methods mainly use cuttings, grafting and other propagation methods, but these propagation methods require a large number of mother plants on the one hand, and on the other hand, they also need to spend a lot of manpower, material resources, financial resources, time, etc., and the reproduction coefficient is low. , the seedlings are slow. With the wide application of cherry blossoms in urban and township landscaping construction, large-scale seedlings are needed. Therefore, traditional breeding methods are far from being able to meet the needs of production. [0003] With the development of modern biotechnology, people use the totipoten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 罗桂杰刘博陈芬
Owner SUQIAN AGRI SCI RES INST JIANGSU ACAD OF AGRI SCI
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