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Rapid seedling growing method of Spiranthes sinensis seeds

A fast and rapid technology, applied in the field of plant tissue culture, can solve problems such as seedling shortage, achieve the effects of reducing deformed seedlings, increasing pH value, and reducing production costs

Active Publication Date: 2018-10-19
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thereby the present invention has solved the difficult problem of seedling shortage in the artificial cultivation of sagegrass

Method used

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  • Rapid seedling growing method of Spiranthes sinensis seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A method for rapidly growing seedlings of sagegrass seeds, said method comprising the following steps in sequence:

[0051] (1) Collection stage of explants

[0052] From May to June every year, collect the fruit ears of Saffron with well-developed capsules for subsequent use;

[0053] (2) Bagging

[0054] The capsule that step (1) gathers and the seed detached from the capsule are packed in the gauze sterilization bag;

[0055] (3) Sterilization treatment

[0056] After the gauze sterilization bag containing the capsules and the seeds detached from the capsules in step (2) is sterilized, take out the sterilized capsules and the detached seeds from the capsules, and put them into a bag covered with sterile filter paper. Reserve in a sterile container;

[0057] (4) Primary culture stage

[0058] The seeds in the capsules after the aseptic treatment in step (3) are stripped out, and the seeds stripped from the capsules and the seeds separated from the capsules are in...

Embodiment 2

[0074] A method for rapidly growing seedlings of sagegrass seeds, said method comprising the following steps in sequence:

[0075] (1) Collection stage of explants

[0076] From May to June every year, collect the fruit ears of Saffron with well-developed capsules for subsequent use;

[0077] (2) Bagging

[0078] The capsule that step (1) gathers and the seed detached from the capsule are packed in the gauze sterilization bag;

[0079] (3) Sterilization treatment

[0080] After the gauze sterilization bag containing the capsules and the seeds detached from the capsules in step (2) is sterilized, take out the sterilized capsules and the detached seeds from the capsules, and put them into a bag covered with sterile filter paper. Reserve in a sterile container;

[0081] (4) Primary culture stage

[0082] The seeds in the capsules after the aseptic treatment in step (3) are stripped off, and the seeds stripped off from the capsules and the seeds stripped off from the capsules...

Embodiment 3

[0097] Embodiment 3 (preferred embodiment)

[0098] A method for rapid seedling cultivation of Sagegrass, said method comprising the following steps:

[0099] (1) Collection stage of explants

[0100] From May to June every year, collect the fruit ears of Saffron with well-developed capsules for subsequent use;

[0101] (2) Bagging

[0102] The capsule that step (1) gathers and the seed detached from the capsule are packed in the gauze sterilization bag;

[0103] (3) Sterilization treatment

[0104] After sterilizing the bag bagged in step (2) together with the capsules and the seeds separated from the capsules, take out the sterilized capsules and the seeds separated from the capsules, and put them into a sterile container covered with sterile filter paper Internal reserve;

[0105] In step (1) in the stage of explant collection, the well-developed capsules are capsules with yellowish pods that are about to split or have just split.

[0106] When bagging in step (2), ev...

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Abstract

The invention relates to a Spiranthes sinensis reproduction method, especially relates to a rapid seedling growing method of Spiranthes sinensis, and belongs to the technical field of plant tissue culture. The method sequentially comprises the following steps: (1) collecting: collecting Spiranthes sinensis clusters of capsules, for later use, from May to June every year; (2) bagging: adding the capsules and seeds into a sterilizing bag; (3) sterilizing: sterilizing the capsules and seeds by the sterilizing bag, and placing the sterilized capsules and seeds, for later use, in a sterile container; (4) carrying out primary culturing: culturing the seeds until the seeds are germinated to form protocorms or budlets; and (5) carrying out subculture seedling formation: inoculating a subculture seedling growing and rooting medium with the protocorms or budlets, and subculturing the protocorms or budlets. The rapid seedling growing method of Spiranthes sinensis seeds adopts the Spiranthes sinensis seeds as a reproduction material to optimize the seedling growing step; and dedifferentiation and re-differentiation of cells are avoided, and seedlings are all developed from embryos, so the formed seedlings are uniform, and the problem of shortage of seedlings in artificial cultivation of Spiranthes sinensis is solved.

Description

technical field [0001] The invention relates to a method for propagating sagegrass, in particular to a method for rapid seedling cultivation of sagegrass, and belongs to the technical field of plant tissue culture. Background technique [0002] Spiranthes sinensis (Pers.) Ames. is a perennial herbaceous plant belonging to the genus Spiranthes of Orchidaceae. The plant is 15-50cm high, with several roots, finger-like, fleshy, clustered at the base of the stem. The stem is short, with 2-4 leaves near the base. Leaves linear or linear oblanceolate, 3.5-15 cm long, usually 3-12 mm wide, apex acuminate to obtuse, base cuneate. Flower stems terminal, 5-20 cm long, glandular pilose to glabrous at the upper part; racemes with numerous dense flowers, 4-10 cm long, spirally twisted; flower bracts ovate-lanceolate, apex long Acuminate, lower part longer than ovary; ovary fusiform, twisted, glandular pilose, pedicels 4-5 mm long; flowers small, purplish red, pink or white, spirally a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘保财陈菁瑛黄颖桢赵云青
Owner 福建省农业科学院农业生物资源研究所
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