Genetic group for molecular subtyping of medulloblastoma and use of SNCA gene as biomarker of 4-type medulloblastoma

A technique for molecular typing of medulloblastoma, applied in the new application field of SNCA gene, can solve the problem of not further providing potential molecular targets for gene targeted therapy, limiting the application prospects of molecular markers, lack of high specificity and high sensitivity Diagnostic markers and other issues

Active Publication Date: 2018-11-02
常青
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method still has certain limitations: the gene combination is limited to diagnostically distinguishing the 4 molecular subtypes in MB, but it does not provide further information on potential molecular targets for gene-targeted therapy and judgment of patient prognosis; At the same time, the molecular markers provided by this combination are only b

Method used

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  • Genetic group for molecular subtyping of medulloblastoma and use of SNCA gene as biomarker of 4-type medulloblastoma
  • Genetic group for molecular subtyping of medulloblastoma and use of SNCA gene as biomarker of 4-type medulloblastoma
  • Genetic group for molecular subtyping of medulloblastoma and use of SNCA gene as biomarker of 4-type medulloblastoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Classification of Medulloblastoma and Screening of Related Marker Genes of Specific Subtypes

[0063] The FFPE samples of 12 medulloblastoma patients were randomly obtained, and the RNA was extracted with a commercially available kit and the RNA concentration was determined.

[0064] Using the nanoString nCounter technology platform (internal standard genes are CLTC, GAPDH and TUBB), the extracted RNA was detected for the following genes:

[0065] Table 3. Candidate genes

[0066]

[0067]

[0068] Select 12 genes (3 for each subtype) from the 22 recognized molecular subtype-specific genes, and conduct preliminary judgment of MB molecular subtype by Nanostring detection. On this basis, further detect the above 89 genes in MB. The expression of MB molecular subtypes is screened out, and the genes related to MB molecular subtypes that may become molecular therapeutic targets are selected, and other technical methods (DNA next-generation sequencing, gen...

Embodiment 2

[0069] Example 2 NanoString typing and immunohistochemical staining of formaldehyde-fixed paraffin-embedded samples

[0070] Using the 11 tumor-associated genes screened in Example 1 and the known 22 genes associated with MB molecular subtypes (WIF1, TNC, GAD1, DKK2, EMX2, PDLIM3, EYA1, HHIP, ATOH1, SFRP1, IMPG2, GABRA5, EGFL11, NRL, MAB21L2, NPR3, KCNA1, EOMES, KHDRBS2, RBM24, UNC5D, OAS1) constitute a gene group of 33 genes, and the gene group of 22 genes is known to carry out MB sample analysis based on the Nano-String nCounter analysis platform For type comparison, the nanoString nCounter detection probes for each gene are shown in Table 2 above.

[0071] Formaldehyde-fixed paraffin-embedded samples (FFPE samples) were obtained from 106 medulloblastoma (MB) patients from the Third Hospital of Peking University, Beijing Tiantan Hospital, Beijing Sanbo Brain Hospital, and Anhui Provincial Hospital. Total RNA was extracted from slices of 75 MB samples and analyzed based o...

Embodiment 4

[0093] Example 4 Knockout and overexpression of SNCA in MB cells

[0094] Daoy, D283 and D341 cells (from ATCC) were 4 × 10 4 The cell density was seeded in a 6-well plate, and any one of the three SNCA siRNAs (siRNA1, siRNA2, siRNA3) was transfected into the cells using Chemifect transfection reagent (Feng Rui, China) according to the manufacturer's instructions, with a final concentration of 20μmol / L.

[0095] With GV219 ( Figure 4 4A) in ) is the backbone, and insert the SNCA gene fragment through the restriction site XhoI / KpnI using conventional methods in the art to construct the plasmid GV219-SNCA-wt. The constructed GV219-SNCA-wt was used as a template, and the following sequences were used as forward primer and reverse primer respectively for amplification:

[0096] SEQ-F (769-789bp): CGCAAATGGGCGGTAGGCGTG

[0097] SEQ-R (1101-1121bp): CCCACTGTCCTTTCCTAATAA

[0098] The verified sequence contains the SNCA gene sequence ( Figure 4 The italic part of 4B), whic...

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Abstract

The invention discloses a genetic group for molecular subtyping of medulloblastoma. The genetic group is formed on one or more of 32 genes in total. The invention further discloses a kit for molecularsubtyping of medulloblastoma and use of the genetic group in the preparation of a reagent for molecular subtyping of medulloblastoma. Besides, the invention further provides use of an SNCA gene for diagnosis or as a diagnosis marker of 4-type medulloblastoma.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of Chinese Patent Application No. 201710260539.4 filed on April 20, 2017, the entire contents of which are hereby incorporated by reference. technical field [0003] The invention belongs to the field of biotechnology, and in particular, the invention relates to a gene group that can be used for molecular typing of medulloblastoma and its use, as well as a new use of the SNCA gene. Background technique [0004] Medulloblastoma (MB) is a common malignant cerebellar embryonal tumor in children. With the deepening of research, it has been found that medulloblastoma is not a single disease, and it is currently recognized that it can be divided into four main molecular subtypes, namely Wnt type (Wnt sungroup), Shh type (Sonic hedgehog subgroup), Shh type (Sonic hedgehog subgroup), Type 3 (type 3) and type 4 (type 4), these molecular subtypes have different characteristics in terms of tr...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/12
CPCC12Q1/6886C12Q2600/118C12Q2600/156
Inventor 常青
Owner 常青
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