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Preparation for detecting long chain noncoding RNA BC200 and application method of preparation

A long-chain non-coding and detection reagent technology, applied in biochemical equipment and methods, microbial measurement/testing, etc., to achieve important promotion and application prospects and far-reaching clinical significance

Inactive Publication Date: 2018-11-02
CENT SOUTH UNIV
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Problems solved by technology

[0006] Lnc-BC200 has not been studied in EBV-associated nasopharyngeal carcinoma

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  • Preparation for detecting long chain noncoding RNA BC200 and application method of preparation
  • Preparation for detecting long chain noncoding RNA BC200 and application method of preparation
  • Preparation for detecting long chain noncoding RNA BC200 and application method of preparation

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Embodiment Construction

[0043] In previous research work, differentially expressed LncRNAs were screened out by performing RNA-seq chip detection on EBV positive or negative cells ( figure 1 ), based on the analysis of these RNA sequencing technologies and databases, we detected the differential gene status in four EBV stably infected 293 cell lines (293-EBV, 293-1 / NL, C22, C2089) established by our laboratory, DAVID, NCBI, Starbase and other databases were used to analyze RNA-seq data, and the significantly changed genes after EBV stable infection were analyzed for functional cluster enrichment analysis such as GO and KEGG. At the same time, 8 lncRNAs were screened to verify the expression value of RNA-Seq transcriptome sequencing data. Further study their expression in epithelial tumor cells 293-HEK and clinical samples of nasopharyngeal carcinoma, and analyze the regulation effect caused by EBV infection. Among them, Lnc-BC200 etc. ( figure 2 ).

[0044] Collect 20 normal human nasopharyngeal ...

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Abstract

The invention discloses a preparation for detecting long chain noncoding RNA BC200 (Lnc-BC200) and an application method of the preparation. The Lnc-BC200 related to EB virus is used for preparing a preparation for screening and early diagnosis of high-risk population with nasopharyngeal carcinoma. Through research, the expression level of the Lnc-BC200 is upregulated by extracting RNA from nasopharyngeal carcinoma tissues and carrying out reverse transcription and real-time fluorescent quantitative PCR analysis. Compared with expression of the Lnc-BC200 in nasopharyngeal tissues of 20 normalpersons, the expression of the Lnc-BC200 in nasopharyngeal tissues of 48 patients is improved, wherein delta CT is smaller than or equal to 7.51, and P is smaller than 0.0001. Therefore, the Lnc-BC200can be used as the molecular marker for diagnosis and treatment of the nasopharyngeal carcinoma.

Description

technical field [0001] The invention belongs to the technical field of tumor molecular markers and their applications, and in particular relates to a preparation for detecting long-chain non-coding RNA BC200 and an application method thereof. Background technique [0002] Eptein-Barr virus (EBV) is a member of gamma herpes virus, can infect 90% of human beings, can transform B lymphocytes in vitro, and is closely related to the occurrence and development of nasopharyngeal carcinoma. It expresses various molecules in different stages of tumor development, such as EBNA1-6, three latent membrane proteins LMP1, 2A and 2B, and miRNAs. Epstein-Barr virus latent membrane protein 1 (LMP1) is the main oncoprotein of Epstein-Barr virus, which can induce multiple signaling pathways (such as NF-KB, MAPK kinase (ERK, p38 and JNK), JAK / STAT), causing epithelial cell morphology and Phenotype changes promote epithelial-mesenchymal transition (EMT), leading to tumor cell invasion and metast...

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 卢建红张四维张靖
Owner CENT SOUTH UNIV
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