Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

ENO1 gene promoter region methylation site and application thereof in preparation of kit for early diagnosis and prognosis evaluation of esophageal cancer

A gene promoter region, early diagnosis technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial assay/examination, etc. Good specificity and easy operation

Active Publication Date: 2022-04-29
NANJING DRUM TOWER HOSPITAL
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another study reported that patients with high expression of ENO1 had a lower overall survival rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ENO1 gene promoter region methylation site and application thereof in preparation of kit for early diagnosis and prognosis evaluation of esophageal cancer
  • ENO1 gene promoter region methylation site and application thereof in preparation of kit for early diagnosis and prognosis evaluation of esophageal cancer
  • ENO1 gene promoter region methylation site and application thereof in preparation of kit for early diagnosis and prognosis evaluation of esophageal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Methylation changes of ENO1 gene in esophageal squamous cell carcinoma and its clinicopathological significance

[0072] 1. Preparation of DNA: The tumor tissues of 68 patients with esophageal squamous cell carcinoma and their corresponding 62 adjacent normal tissues were selected, fixed in formalin, and embedded in paraffin. Among them, the paracancerous normal tissues were normal esophageal tissue wax blocks and were selected from samples more than 2 cm away from esophageal squamous cell carcinoma tissues. Genomic DNA was extracted using QIAamp DNA FFPE Tissue Kit, the absorbance value was measured by ultraviolet spectrophotometer to determine its content and purity, and gel electrophoresis was used to ensure the quality of the extracted DNA.

[0073] 2. Bisulfite treatment: use EZ-96DNA Methylation-Gold™ Kit for bisulfite modification. The obtained DNA was used immediately or stored at -20°C for future use.

[0074] 3. PCR amplification reaction: design primers for...

Embodiment 2

[0129] Expression of ENO1 protein in esophageal squamous cell carcinoma tissues and adjacent normal tissues.

[0130] The expression of ENO1 protein was detected in 273 esophageal squamous cell carcinoma tissues and 263 adjacent normal tissues by immunohistochemistry.

[0131] The prepared esophageal cancer tissue microarray wax blocks were taken for 4 μm serial sections, and the immunohistochemical method of the tissue microarray was the same as the conventional method. This experiment adopts immunohistochemical Envision two-step method, the specific steps are as follows:

[0132] (1) Before the experiment, put the prepared 4 μm white slices into the slicing box and store them in a refrigerator at 4°C for subsequent experiments;

[0133] (2) Before the experiment, insert the white chip of the tissue chip stored in the 4°C refrigerator into the metal basket and bake in a 60°C oven for 1.5-2 hours to prevent the tissue from falling off;

[0134] (3) Put the paraffin tissue sl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an ENO1 gene promoter region methylation site and application thereof in preparation of a kit for early diagnosis and prognosis evaluation of esophageal cancer, and belongs to the technical field of disease diagnosis and prognosis rating. A methylation site of an ENO1 gene promoter region is as shown in SEQ ID No.1, DNA of esophageal squamous carcinoma and para-carcinoma normal sample tissues is extracted, the methylation frequency of a CpG site of the ENO1 gene promoter region is detected by adopting flight mass spectrometry after the DNA is modified by hydrosulfite, and meanwhile, expression of ENO1 protein in the esophageal squamous carcinoma and para-carcinoma normal tissue samples is detected by adopting immunohistochemistry; research finds that an ENO1 gene promoter region in esophageal squamous carcinoma is low in methylation and ENO1 protein is high in expression, patients with low methylation are poor in prognosis, and ENO1 can promote glycolysis level of esophageal carcinoma cells, promote proliferation of the esophageal carcinoma cells and inhibit apoptosis of the esophageal carcinoma cells; the invention relates to a reagent for early diagnosis and prognosis evaluation of esophageal squamous carcinoma by detecting the methylation level of an ENO1 gene.

Description

technical field [0001] The invention belongs to the technical field of disease diagnosis and prognosis rating, and specifically relates to a methylation site in the promoter region of ENO1 gene and its application in the preparation of a kit for early diagnosis and prognosis evaluation of esophageal cancer. Background technique [0002] Esophageal cancer (Esophageal carcinoma, EC) is the sixth leading cause of cancer-related deaths worldwide. Esophageal squamous cell carcinoma (ESCC) is the most common histological subtype of esophageal cancer, especially in East Asia, East Africa and South Africa. In China, about 90% of esophageal cancer patients are esophageal squamous cell carcinoma. Esophageal cancer in China has obvious geographical distribution, and the incidence rate of all tumors ranks first in Henan, Hebei, Jiangsu, Shaanxi, Shanxi, Fujian and other places. Despite improvements in diagnosis and treatment methods, the survival rate of patients with advanced ESCC is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154C12Q2600/158C12Q2600/118
Inventor 崔晓宾赵琳玥王文洁陈云昭孙琦丁友祥禹洁陈曦李锋韩雪萍孟凡青
Owner NANJING DRUM TOWER HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com