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Application of SLIT3 in preparation of kit for prognosis of lung adenocarcinoma surgery patient

A kit, the technology of lung adenocarcinoma, applied in the field of biomedicine

Pending Publication Date: 2020-11-27
GUANGXI MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a comprehensive analysis of the clinicopathological and predictive significance of SLIT3 in cancer, especially in LUAD, is still lacking

Method used

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  • Application of SLIT3 in preparation of kit for prognosis of lung adenocarcinoma surgery patient
  • Application of SLIT3 in preparation of kit for prognosis of lung adenocarcinoma surgery patient
  • Application of SLIT3 in preparation of kit for prognosis of lung adenocarcinoma surgery patient

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Embodiment Construction

[0026] 1. Materials and methods

[0027] Lung adenocarcinoma tissues and paired noncancerous tissues (at least 5 cm from the tumor margin) were collected prospectively from patients with lung adenocarcinoma who underwent surgical resection at the Cancer Hospital Affiliated to Guangxi Medical University from December 2018 to May 2019. Selection criteria included: 1) pathological diagnosis of LUAD; 2) patients did not receive chemotherapy or radiotherapy before surgery; 3) patients had no history of other types of tumors; 4) patients had complete medical records. Patients who lacked AJCC staging information, lacked histological information, and died within 30 days after surgery or from other causes of disease were excluded from the analysis. Human tissues for research were reviewed and approved by the Ethics Review Committee of Guangxi Medical University for research involving human subjects.

[0028] 2. RNA extraction, quantification and identification

[0029] RNA was extrac...

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Abstract

The invention discloses application of SLIT3 in preparation of a kit for prognosis of a lung adenocarcinoma surgery patient. The inventor identifies differentially expressed genes (DEGs) between 13 lung adenocarcinoma tissues and 5 normal lung tissues after radical operation through RNA sequencing (RNA-seq), analyzes by using gene ontology (GO) and KEGG, confirms expression of DEGs in a clinical specimen, a lung adenocarcinoma cell line, a normal human bronchial epithelial cell line and a verification queue from GEPIA through qRT-PCR, and verifies through survival curve analysis, so that the SLIT3 can be used as a biomarker for predicting the prognosis condition of the lung adenocarcinoma surgery excision patient.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of SLIT3 in the preparation of a kit for the prognosis diagnosis of lung adenocarcinoma patients. Background technique [0002] Lung cancer is the leading cause of cancer-related death worldwide. Among the various pathological types of lung cancer, lung adenocarcinoma (LUAD) accounts for about 40% of lung cancer and is the most common histopathological subtype of lung cancer. Its morbidity and mortality are on the rise. The vast majority of patients are diagnosed at an advanced stage with local and distant metastatic dissemination, limiting surgical options for patients with lung adenocarcinoma. However, the prognosis of patients with early-stage lung adenocarcinoma is poor even after radical surgical resection. According to statistics, about 50% of patients have tumor recurrence or metastasis within 5 years after surgery, most of which occur within 1 year a...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/118
Inventor 阳洁宋锦静韦彤彤沈小菊黎骊莫小香莫孝成
Owner GUANGXI MEDICAL UNIVERSITY
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