Preparation method of inactivated vaccine for preventing duck reovirus

Abstract

The invention is applicable to the technical field of vaccine preparation, and provides a preparation method of inactivated vaccine for preventing duck reovirus. The method comprises the following steps: step 1, selecting standard viral species; step 2, preparing the standard viral species into viral species for production; step 3, preparing an antigen preparation material and preparing antigen according to the viral species for production; step 4, detecting the antigen and performing concentration inactivation on the antigen. The method has the advantages that inoculation is only required tobe performed before breed ducks start laying, the inoculation dosages of two vaccines are respectively 1ml and 0.8ml for one duck, after vaccination is performed about 21 days, hatching eggs can be collected for hatching, by taking that the protection rate of duck reovirus of ducklings is greater than 98 percent as a standard, hatching egg utilization cycles of the two vaccines after vaccination are respectively 6 weeks and 9 weeks after vaccination, immune vaccines are enhanced again after 6 weeks and 9 weeks after vaccination of the two vaccines, and the dosages are the same, the vaccines are repeatedly inoculated till the breed ducks are eliminated, and the preventive effect on liver and spleen necrosis caused by infection of the duck reovirus of the two vaccines with two types of adjuvant are exact.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, in particular to a method for preparing an inactivated vaccine for preventing duck reovirus. Background technique [0002] Since March 2017, a kind of duckling has been popular in the farms of large-scale meat duck breeding groups in China. The clinical symptoms are soft feet, loose stools, and the pathological changes are yellowish necrosis in the liver and occasional bleeding points, and gray-white necrosis in the spleen. Or spleen enlargement and purple-black disease, clinically known as duck "liver and spleen necrosis". Cherry Valley ducks are more prone to occur, the age of onset is mostly within 9 days, the incidence rate is less than 30%, and the mortality rate within 7 days after the onset is about 10%. [0003] At present, except for the use of refined yolk antibody against duck reovirus to inject 0.5-0.8ml per duckling at the age of 1-5 days to prevent "liver and spleen nec...

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Problems solved by technology

[0004] The invention provides a method for preparing an inactivated vaccine for preventing duck reovirus, aiming at solving the problem of injecting 0.5-0.8ml / ducklings into ducklings at the age of 1-5 days with refined egg yolk antibodies against duck reovirus In addition to preventing "liver and spleen necrosis" caused by duck reovirus, there is no vaccine to prevent duck reovirus. In addition, each duckling needs to be injected with refined egg yolk antibody to duck reovirus. Large amount, high cost, and improper inoculation operation can easily lead to the death of ducklings

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