Method for performing tissue source tracing based on target gene capture technology of free DNA

Abstract

The invention discloses a method for performing tissue source tracing based on a target gene capture technology of free DNA. The method comprises the following steps that 1, single-chain linkers and double-chain linkers are utilized to establish a free DNA library; 2, target genes are captured from the library, and captured target genes are sequenced to obtain sequencing results, wherein the target genes are a part of whole genomes; 3, the sequencing results are compared with human genomes to obtain comparison results; 4, a reference library is established, wherein the reference library includes a variety of different reference samples, the reference samples include multiple different genes, and a variety of different genes have corresponding FPKM gene expression values; 5, based on the reference library, the comparison results are screened, and the association between the screened results and the FPKM gene expression values is calculated, and tissue source tracing is performed based on the association.

Description

technical field [0001] The invention relates to the field of gene analysis, in particular to a method for tissue traceability based on free DNA single-strand library construction combined with target gene capture technology. Background technique [0002] With the increasing clinical demand for liquid biopsy and non-invasive monitoring, the research of cell-free DNA (cfDNA: cell-freeDNA) in cancer is becoming more and more important. It is well known that cfDNA exists in circulating plasma, urine and other body fluids in the human body (Cell-free nucleic acids in plasma, serum and urine: a new tool inmolecμlar diagnosis. Ann. Clin. Biochem. 40, 122-130), and in healthy In individuals, cfDNA is considered to be mainly derived from the apoptosis of normal cells of the hematopoietic line, while other tissues contribute very little to it (Predominanthematopoietic origin of cell-free DNA in plasma and serum after sex-mismatched bone marrow transplantation.Clin.Chem.48,421-427 ). ...

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Problems solved by technology

[0004] In order to solve the above problems, the present invention provides a method for tissue traceability, which does not require deep sequencing of the entire genome of an individual's cfDNA, but only requires target capture and sequencing of specific genes to achieve the purpose of tissue traceability, which not only greatly saves The amount of sequencing data reduces the cost of sequencing and the complexity of subsequent analysis, and provides flexible gene combination options to meet the needs of specific individual tissue traceability

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