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Method for counting reticulocyte by using improved Neubauer counting plate

Inactive Publication Date: 2018-11-16
CANGZHOU MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, due to its high price and complicated operation, the Miller peeping method is basically not used in most laboratories in China.

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  • Method for counting reticulocyte by using improved Neubauer counting plate
  • Method for counting reticulocyte by using improved Neubauer counting plate
  • Method for counting reticulocyte by using improved Neubauer counting plate

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Embodiment Construction

[0022] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0023] A method for counting reticulocytes with an improved Neubauer counting board of the present invention comprises the following steps:

[0024] (1) Preparation of cell suspension: Take a small test tube, add 100 μl of blood, add 100 μl of 1% brilliant tar blue saline staining solution, mix well, let stand in a constant temperature water bath at 37°C for 10 minutes, and shake well; normal saline can also be used 100-fold dilution, direct reticulocyte count;

[0025] (2) Select a reticulocyte counting plate: wipe the modified Neubauer counting plate and cover glass clean, cover the cover glass on the improved Neubauer counting plate, use a micropipette to draw the mixed cell suspension into the improved ...

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Abstract

The invention relates to the technical field of reticulocyte counting, in particular to a method for counting reticulocyte by using an improved Neubauer counting plate. According to the method, the normalized operation for directly counting the reticulocyte by using the improved Neubauer counting plate is established, the result of the method is consistent with a method approved by the authority,and a satisfactory effect is achieved; the trouble of preparing a blood smear by using a Miller ocular is reduced, and the error caused by nonuniform distribution of the reticulocyte is avoided; the operation procedures are simplified, and the method has the advantages of being simple, direct, low in price, fast, practical, accurate, reliable, capable of realizing normalized and standardized operation and capable of being widely popularized in clinic; and the method has great significance in anemia diagnosis, differential diagnosis, curative effect observation, prognosis monitoring and the judgment of the success of bone marrow transplantation. The method comprises the following steps of (1) preparing a cell suspension; (2) selecting the reticulocyte counting plate; and (3) carrying out counting.

Description

technical field [0001] The invention relates to the technical field of counting reticulocytes, in particular to a method for counting reticulocytes with an improved Neubauer counting board. Background technique [0002] Reticulocytes are the transitional type between late immature red blood cells and immature red blood cells. Due to the residual basophilic substances such as ribosomes in the cytoplasm, after living staining with brilliant tar blue or new methylene blue dyes , The blue reticulum is located in the cytoplasm of red blood cells, so it is called reticulocytes. [0003] Reticulocytes are usually manually counted under a microscope. In addition, there are flow cytometry and hematology analyzer methods, but they all require expensive instruments and are not suitable for primary hospitals. Manual counting methods include glass slide method, test tube method and Miller peeping disk method. Mix blood and dye in a glass slide or test tube, stain at room temperature at...

Claims

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Application Information

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IPC IPC(8): G01N15/10
CPCG01N15/10G01N2015/1006G01N2015/014G01N2015/1024
Inventor 侯振江陈洋刘玉枝李红岩王凤玲
Owner CANGZHOU MEDICAL COLLEGE
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