CYP703A3 gene based cultivation method for transgenic rice sterile line

Abstract

The application discloses a CYP703A3 gene based cultivation method for a transgenic rice sterile line, which comprises the steps as follows: A, acquisition of a rice gene cassette CYP703A3; B, acquisition of an sfGFP gene cassette; C, amplification of a wheat pollen lethal gene Ki; D, amplification of a wheat pollen specific promoter Pg47; and E, connection of each gene expression element. The step E of connection of each gene expression element comprises: firstly, introducing a complete rice CYP703A3 gene into a pCAMBIA1300 vector; and then connecting the sfGFP gene cassette, the wheat pollenlethal gene Ki and the wheat pollen specific promoter pPg47 to the vector. According to the invention, a F1-generation heterozygote follows the Mendel segregation law in the self fruitfulness process, and generated progenies not only contain heterozygotes which can keep a triple linked gene, but also contain the sterile line without fertility.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering genetics and breeding, in particular to a method for cultivating a transgenic rice sterile line based on CYP703A3 gene. Background technique [0002] Rice is native to tropical Asia, and after being widely planted in China, it gradually spread to all parts of the world. Rice is the staple food of nearly half of the world's population. In addition to being edible, rice can also be used for wine making, sugar refining and as an industrial raw material. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hyb...

AI Technical Summary

Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low

However, the rice CYP703A3 gene mutation will cause the tapetum cells to fail to degrade in time, and the programmed death will be delayed, so the callose enzyme secreted by the tapetum will affect the development and separation of microspores, and finally show sterility

Images