Four-laser device for capturing particles or cells through optical tweezers

Abstract

The invention discloses a four-laser device for capturing particles or cells through optical tweezers, and the device comprises a pedestal, an objective table, and a lens cone. One side of the pedestal is provided with a support arm, and the support arm is connected with the objective table and the lens cone. The particles or cells are disposed on the objective table, and the objective table is provided with an avoiding hole. A light transmitting plate connected with the particles or cells is disposed on the avoiding hole, and the bottom of the light transmitting plate is provided with opticaltweezers equipment connected with the pedestal. The optical tweezers equipment comprises a movable platform, a light source which is disposed on the movable platform, and four optical tweezers emitters, wherein the generated laser tweezers can pass through the particles or cells on the light transmitting plate to be focused around the particles or cells. Compared with the conventional optical tweezers technique, the device provided with the four optical tweezers emitters, and the volume and mass of the extracted cells are 30-40 times of the volume and mass of the cells extracted through the common optical tweezers technique, and the drive, control and separation effects of a plurality of particles are acheived.

Description

technical field [0001] The invention relates to the technical field of optical tweezers, in particular to a device for capturing particles or cells by optical tweezers with four lasers. Background technique [0002] In the fields of life science, chemistry, and nanotechnology, it is necessary to perform various manipulations and tests on tiny objects, such as cell nuclei, DNA chains, molecular groups, and micro-mechanical structures, such as handling, cutting, peeling, grafting, injection, assembly, and curing. , activation, fluorescence detection, electrophoresis detection, etc. Common microscopic manipulation methods include mechanical probes, molecular tweezers, optical tweezers, etc., in which mechanical probes can puncture cells; molecular tweezers can adsorb cells on the front of capillaries to quantitatively measure cell membrane Ion channels; optical tweezers can capture and move tiny particles by relying on the sharply changing gradient light field in the focused sp...

AI Technical Summary

Problems solved by technology

[0007] The entire sorting process of this method is complicated to operate. When the surface of the cells to be sorted does not contain antigens or the cells to be sorted are mutant individuals or the cells to be sorted are bacteria, this method cannot realize the cell sorting function.

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