Device provided with seven laser optical tweezers for capturing particles or cells

Abstract

The invention discloses a device provided with seven laser optical tweezers for capturing particles or cells. The device includes a lens barrel, a moving stage, a base and an optical tweezers device.A light-transmitting plate is arranged in the middle of the moving stage. A particle or cell chamber is arranged on the light transmitting plate. The optical tweezers device comprises a first opticaltweezers device disposed on the lens barrel and a second optical tweezers device disposed on the base. The first optical tweezers device includes a fixed seat connected to the lens barrel and a firstoptical tweezers emitter disposed on the fixed seat, and the second optical tweezers device includes a lifting chassis connected to the base, a rotating chassis disposed on the lifting chassis, and asecond optical tweezers emitter and a third optical tweezers emitter disposed on the rotating chassis. Compared with a conventional optical tweezers technology, the seven laser optical tweezers provided by the invention can extract cells with cell volume and cell mass 60 to 70 times of that of the common optical tweezers technology and simultaneously achieves the optical tweezers effect of driving, controlling or separating multiple particles.

Description

technical field [0001] The invention relates to the technical field of optical tweezers, in particular to a device for capturing particles or cells by optical tweezers with seven lasers. Background technique [0002] In the fields of life science, chemistry, and nanotechnology, it is necessary to perform various manipulations and tests on tiny objects, such as cell nuclei, DNA chains, molecular groups, and micro-mechanical structures, such as handling, cutting, peeling, grafting, injection, assembly, and curing. , activation, fluorescence detection, electrophoresis detection, etc. Common microscopic manipulation methods include mechanical probes, molecular tweezers, optical tweezers, etc., in which mechanical probes can puncture cells; molecular tweezers can adsorb cells on the front of capillaries to quantitatively measure cell membrane Ion channels; optical tweezers can capture and move tiny particles by relying on the sharply changing gradient light field in the focused s...

AI Technical Summary

Problems solved by technology

[0007] The entire sorting process of this method is complicated to operate. When the surface of the cells to be sorted does not contain antigens or the cells to be sorted are mutant individuals or the cells to be sorted are bacteria, this method cannot realize the cell sorting function.

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