Double-laser device for capturing particles or cells through optical tweezers

Abstract

The invention discloses a double-laser device for capturing the particles or cells through optical tweezers, and the device comprises a device body. The device body comprises a microscope and an optical tweezers device disposed on the microscope. The microscope comprises a pedestal, an objective table and a lens cone from the bottom to the top. The bottom of the lens cone is provided with an objective lens, and the top is provided with an ocular lens. A particle or cell chamber is disposed below the objective lens, and is disposed on the objective table. The optical tweezers device comprises afirst optical tweezers transmitter and a second optical tweezers transmitter, which are disposed on the pedestal. The laser light generated by the first and second optical tweezers transmitters passthrough a light transmission hole in the objective table to be focused at two sides of the particles or cells behind the particle or cell chamber. Compared with the cell volume and mass extracted through the conventional optical tweezers technology, the cell volume and mass extracted by the device provided with the two optical tweezers transmitters are 3-5 times of the cell volume and mass extracted through the conventional optical tweezers technology, and the device achieves the optical tweezers effects of driving, controlling or separating of the particles.

Description

technical field [0001] The invention relates to the technical field of optical tweezers, in particular to a device for capturing particles or cells by optical tweezers with dual lasers. Background technique [0002] In the fields of life science, chemistry, and nanotechnology, it is necessary to perform various manipulations and tests on tiny objects, such as cell nuclei, DNA chains, molecular groups, and micro-mechanical structures, such as handling, cutting, peeling, grafting, injection, assembly, and curing. , activation, fluorescence detection, electrophoresis detection, etc. Common microscopic manipulation methods include mechanical probes, molecular tweezers, optical tweezers, etc., in which mechanical probes can puncture cells; molecular tweezers can adsorb cells on the front of capillaries to quantitatively measure cell membrane Ion channels; optical tweezers can capture and move tiny particles by relying on the sharply changing gradient light field in the focused sp...

AI Technical Summary

Problems solved by technology

[0007] The entire sorting process of this method is complicated to operate. When the surface of the cells to be sorted does not contain antigens or the cells to be sorted are mutant individuals or the cells to be sorted are bacteria, this method cannot realize the cell sorting function.

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