A kind of medicinal composition for relieving cough and resolving phlegm
A technology for relieving cough and reducing phlegm and composition, which is applied in the field of pharmaceutical composition for relieving cough and reducing phlegm and its preparation, which can solve the problem that the therapeutic effect is no longer obvious, and achieve the effect of significant efficacy, fast onset and no side effects
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[0035] 1.3.2 Preparation of mouse tracheal secretions
[0036] 45 minutes after the last administration, 0.5 ml of phenol red was injected intraperitoneally, and 15 minutes later, the rats were killed by neck dislocation and the trachea was separated. Aspirate 1.5ml NaHCO3, ultrasonically wash, then carefully lavage the inner wall of the trachea with a syringe, and centrifuge at 3000 rpm / 10min to obtain the mouse tracheal secretion.
[0037] 1.3.3 Drawing of standard curve and detection of phenol red excretion
[0038] (1) Configuration of standard products: Dilute phenol red with physiological saline to 0, 10, 20, 40, 80, 120, 160 μg / ml for drawing a standard working curve.
[0039] (2) According to 100μl / well, add the standard substance and the sample to be tested respectively into the 96-well plate according to a certain order.
[0040] (3) Measure the absorbance at 546 nm with a microplate reader.
[0041] (4) Calculate the concentration: calculate the regression equati...
Embodiment 1
[0108] Orange 300g, Scutellaria baicalensis 400g, Golden buckwheat 400g, Baibu 250g, perilla seed 200g, seabuckthorn 200g, aster 200g, bellflower 200g
[0109] Take scutellaria baicalensis, perilla seeds and basil, add 8 times and 6 times the volume of 95% ethanol, reflux extraction twice, each time for 2 hours, filter, combine the filtrates, recover ethanol, concentrate under reduced pressure to a relative density of 1.25 -1.30 thick ointment, dregs for later use; take Huajuhong, seabuckthorn, aster, platycodon, golden buckwheat and the first three medicinal dregs and decoct them with 8 times and 6 times the amount of water respectively, for 2 hours each time, extract The liquid is filtered, concentrated under reduced pressure to a thick paste with a relative density of 1.25-1.30, combined with the above thick paste, and dried under reduced pressure to obtain a dry paste, which is prepared into granules according to conventional methods.
Embodiment 2
[0111] 400g orange red, 450g skullcap, 450g golden buckwheat, 200g basil, 225g perilla seed, 175g seabuckthorn, 250g aster, 150g bellflower
[0112] Take scutellaria baicalensis, perilla seeds and basil, add 8 times the volume of 95% ethanol, reflux extraction twice, each time for 1.5 hours, filter, combine the filtrates, recover ethanol, concentrate under reduced pressure to a relative density of 1.25-1.30 Thick ointment and medicinal dregs for later use; separately take Huajuhong, seabuckthorn, aster, platycodon, golden buckwheat and the first three medicinal dregs, decoct and extract with 8 times the amount of water respectively, each time for 1.5 hours, filter the extract, and decompress Concentrate to a thick paste with a relative density of 1.25-1.30, combine with the above thick paste, and dry under reduced pressure to obtain a dry paste, which is prepared into granules according to a conventional method.
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