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Application of tribulus terrestris in preparation of medicines for preventing and treating retinal injury diseases

A technology for retinal damage and retinal pigment, which is used in sensory diseases, drug combinations, cardiovascular system diseases, etc.

Active Publication Date: 2018-12-07
HE UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There are no relevant reports at home and abroad on the effect of Tribulus terrestris on retinal pigment epithelial cells under oxidative stress. Whether Tribulus terrestris may delay the occurrence and development of AMD by reducing the oxidative stress damage of retinal pigment epithelial cells remains to be studied.

Method used

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  • Application of tribulus terrestris in preparation of medicines for preventing and treating retinal injury diseases
  • Application of tribulus terrestris in preparation of medicines for preventing and treating retinal injury diseases
  • Application of tribulus terrestris in preparation of medicines for preventing and treating retinal injury diseases

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Experimental program
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Effect test

Embodiment 1

[0026] The preparation of embodiment 1 Tribulus terrestris ethanol extract

[0027]Method: According to the ratio of material to liquid 1g:10mL, take 20g of Tribulus terrestris and 200mL of 70% ethanol, reflux extraction at 80°C, filter, take the filtrate, extract twice for 1.5h each time, combine the filtrate, and pass the filtrate through a macroporous adsorption resin column , and then eluted with water and 95% ethanol successively, the water eluted solution was discarded, the 95% ethanol eluted solution was collected, and the ethanol was recovered by decompression to obtain 1.36 g of the ethanol extract of Tribulus terrestris.

Embodiment 2

[0029] The detection method of MTT is as follows: inoculate cells: prepare a single cell suspension with a culture solution containing 10% fetal calf serum, inoculate 1000 cells in each well into a 96-well plate, and the volume of each well is 100ul. After 24 h, the cells were subjected to H 2 o 2 Modeling or administration of Tribulus terrestris. Coloring: Add 20ul of MTS solution to each well. Continue to incubate for 2-4h. Colorimetry: Select a wavelength of 490nm, measure the light absorption value of each well on the enzyme-linked immunosorbent monitor, record the results, draw the cell growth curve with time as the abscissa and the light absorption value as the ordinate.

[0030] The inhibition rate was calculated according to the following formula:

[0031]

[0032] The detection method of flow cytometry is as follows: cells in the logarithmic phase of growth are digested and centrifuged to make a single cell suspension, and the cell density is 1×10 4 / mL inocula...

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Abstract

The invention discloses an application of tribulus terrestris in preparation of medicines for preventing and treating retinal injury diseases. The application disclosed by the invention has the beneficial effects that an anti-apoptotic effect of the tribulus terrestris on retinal pigment epithelial cells injured oxidatively and the molecular action mechanism of the tribulus terrestris are discussed by observing the protective effect of the tribulus terrestris on the retinal pigment epithelial cells induced by hydrogen peroxide (H2O2) and injured by oxidative stress and observing the expressionchange of the tribulus terrestris on the retinal pigment epithelial cells treated by H2O2 and cell-apoptosis related molecules so as to provide theoretical basis for utilizing the tribulus terrestristo prevent and treat eye diseases related to injury of the retinal pigment epithelial cells in future.

Description

technical field [0001] The present invention relates to the pharmacological action and molecular action mechanism of a natural traditional Chinese medicine alcohol extract, in particular to the research and development of tribulus alcohol extract as a candidate health product or new drug for the prevention and treatment of retinal damage diseases in the future, and for the prevention of ophthalmology-related diseases. and clinical treatment to develop new methods and ideas. Background technique [0002] The retinal pigment epithelium (RPE) has complex biological functions, including providing nutrients to the outer cells of the sensory retina, phagocytosis and digestion of the outer segmental membrane of photoreceptor cells, and maintaining metabolism. RPE adheres very closely to the glass membrane (Bruch membrane) in the innermost layer of the choroid, and forms a unified functional unit together with the choriocapillary layer, that is, the RPE-glass membrane-choriocapillar...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P27/02A61P9/10
CPCA61K36/185A61K2236/333A61K2236/51A61K2236/55A61P9/10A61P27/02
Inventor 张东蕾袁振丽何向东何伟
Owner HE UNIV
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