Cultivation method of transgenic rice sterile line based on MIL1 gene

A technology of transgenic rice and breeding methods, which is applied in the fields of genetic engineering, plant genetic improvement, chemical instruments and methods, etc., can solve the problem of low frequency of wild rot maintainer lines, small genetic differences between sterile lines, and spontaneous Issues such as delivery

Inactive Publication Date: 2018-12-07
QINGDAO YUANCE GRP CO LTD
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Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low
[0004] The genetic difference between the existing sterile lines in production is small, the fertility stability of hybrids is not enough, and t

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  • Cultivation method of transgenic rice sterile line based on MIL1 gene
  • Cultivation method of transgenic rice sterile line based on MIL1 gene
  • Cultivation method of transgenic rice sterile line based on MIL1 gene

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Embodiment Construction

[0034] The present invention is described in detail below in conjunction with embodiment. In order to make the object, technical solution and advantages of the present invention clearer and clearer, the present invention will be described in further detail below with reference to the accompanying drawings and examples, but the present invention is not limited to these examples.

[0035] In the invention, the common nuclear sterile gene MIL1 of rice is linked with the lethal gene Ki of wheat pollen, and at the same time, the red fluorescent protein mCherry gene is used as a reporter gene to carry out genetic modification on the rice MIL1 mutant to obtain the F1 generation hybrid. The hybrids of the F1 generation follow Mendel's segregation law in the process of self-fertilization, and the offspring produced include heterozygotes that can maintain the triple-linkage genes and sterile sterile lines, which are the so-called third-generation sterile lines.

[0036] In order to solv...

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Abstract

The invention discloses a cultivation method of a transgenic rice sterile line based on an MIL1 gene. The cultivation method includes the following steps: A, obtainment of a rice MIL1 gene expressioncassette; B, obtainment of an mCherry gene expression cassette; C, amplification of a wheat pollen specific promoter Pg47; D, amplification of a wheat pollen lethal gene Ki; and E, linkage of all geneexpression elements. The step E which is the linage of all the gene expression elements further includes the following steps: firstly, linking the complete rice MIL1 gene to a pCAMBIA1390 vector; andsecondly, introducing the mCherry gene expression element to the pCAMBIA1390 vector which is introduced with the MIL1 gene in the first step. An F1 generation heterozygote follows the Mendelian separation law in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

Description

technical field [0001] The invention relates to the technical field of molecular biology nucleic acid chemistry, in particular to a method for preparing a genetically engineered sterile line of rice. Background technique [0002] Rice is the staple food of nearly half of the world's population. In addition to being edible, it can also be used for wine making, sugar refining and as an industrial raw material. Humans have a great demand for rice. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hybrid rice is a three-line method using the nuclear-plasma interaction male sterile line as a geneti...

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8289
Inventor 张国栋刘佳音米铁柱万吉丽徐春莹葛序娟
Owner QINGDAO YUANCE GRP CO LTD
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