Cultivation method of transgenic rice sterile line based on MIL1 gene

Abstract

The invention discloses a cultivation method of a transgenic rice sterile line based on an MIL1 gene. The cultivation method includes the following steps: A, obtainment of a rice MIL1 gene expressioncassette; B, obtainment of an mCherry gene expression cassette; C, amplification of a wheat pollen specific promoter Pg47; D, amplification of a wheat pollen lethal gene Ki; and E, linkage of all geneexpression elements. The step E which is the linage of all the gene expression elements further includes the following steps: firstly, linking the complete rice MIL1 gene to a pCAMBIA1390 vector; andsecondly, introducing the mCherry gene expression element to the pCAMBIA1390 vector which is introduced with the MIL1 gene in the first step. An F1 generation heterozygote follows the Mendelian separation law in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

Description

technical field [0001] The invention relates to the technical field of molecular biology nucleic acid chemistry, in particular to a method for preparing a genetically engineered sterile line of rice. Background technique [0002] Rice is the staple food of nearly half of the world's population. In addition to being edible, it can also be used for wine making, sugar refining and as an industrial raw material. Humans have a great demand for rice. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hybrid rice is a three-line method using the nuclear-plasma interaction male sterile line as a geneti...

AI Technical Summary

Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low

[0004] The genetic difference between the existing sterile lines in production is small, the fertility stability of hybrids is not enough, and the ability to resist adversity is poor

However, in the "two-line method", the fertility of the photothermosensitive male sterile line is controlled by the external temperature, which may easily lead to the self-fertilization of the photothermosensitive male sterile line and the failure of seed production.

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