Method for rapidly separating cuticle of plant leaf

A technology for plant leaves and cuticles, applied in the separation of plant leaves cuticles, in the biological field, can solve the problems of destroying the integrity of the inner surface of the cuticle, difficult to distinguish the inner and outer surfaces of the cuticle, and dangerous operation processes, etc., to achieve good enzymatic hydrolysis effect, The effect of fast operation and safe operation

Inactive Publication Date: 2018-12-11
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above methods are not easy to operate, the operation process is dangerous (the operator needs to be exposed to dangerous chemicals), the processing time is long, the chemical reagent treatment causes the tissue to hydrolyze and destroys the integrity of the inner surface of the stratum corneum, and the inner and outer surfaces of the stratum corneum are not easy. Differentiation, and difficulty in removing cells remaining on the inner surface of the stratum corneum

Method used

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  • Method for rapidly separating cuticle of plant leaf
  • Method for rapidly separating cuticle of plant leaf
  • Method for rapidly separating cuticle of plant leaf

Examples

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Effect test

no. 1 example

[0043] The method for separating plant leaf cuticle rapidly of the present invention carries out according to the following steps:

[0044] 1. Tear off the epidermis: select fresh and green plant leaves, and use tweezers to tear off the epidermis of the leaves;

[0045] 2. Positioning of the inner surface of the stratum corneum: cover the upper and lower surfaces of the epidermis torn off in step 1 with a nylon film, place the inner surface of the epidermis upwards, and obtain the positioning epidermal structure;

[0046] 3. Removal of mesophyll cells: first prepare enzymatic hydrolysis solution, weigh 0.5%-3% cellulase, 0.005%-0.015% polyvinylpyrrolidone, 0.5%-3% bovine serum albumin and Pour 50%-60% of the osmosis buffer into a petri dish, mix to obtain an enzymolysis solution, the pH of the enzymolysis solution is 5-6; add the localized epidermal structure obtained in step 2 to the above enzymolysis solution to carry out Enzymolysis reaction, and ensure that the inner surf...

no. 2 example

[0052] The method for quickly separating the cuticle of plant leaves provided by the embodiment of the present invention is different from the first embodiment in that the pore size of the nylon membrane is 30um-100um.

[0053] Wherein, the cellulase is cellulase R-10, the product number is 6008, and the product parameter is 10000U / g.

[0054] The method for quickly separating the cuticle of plant leaves provided by the embodiment of the present invention is different from the foregoing embodiments in that the diameter of the petri dish is 5ml-15ml.

no. 3 example

[0056] The method for quickly separating the cuticle of plant leaves provided by the embodiment of the present invention is different from the first embodiment and the second embodiment in that the drying method of the sample described in step 5 is the natural drying method and the ultra-clean table air blowing assisted drying method or freeze-drying.

[0057] Wherein, the plants are angiosperms.

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Abstract

The invention discloses a method for rapidly separating cuticle of a plant leaf, belongs to the technical field of biology, and particularly relates to the field of separating the cuticle of the plantleaf. The method for rapidly separating the cuticle of the plant leaf comprises the following steps of 1, tearing of epidermis; 2, positioning of the inner surface of the cuticle; 3, removal of mesophyll cells: firstly, preparing an enzymatic hydrolysate, adding a positioned epidermis structure obtained in step 2 into the enzymatic hydrolysate for enzymatic hydrolysis, and ensuring the inner surface of the positioned epidermis structure to be placed upward, wherein the enzymolysis temperature is 23-27 DEG C, and the enzymolysis time is 2-4 hours; 4, cleaning of the inner surface of the cuticle to obtain a cleaned sample; and 5, drying of the sample: drying the sample obtained in step 4 to obtain the separated cuticle structure. The method for rapidly separating the cuticle of the plant leaf provided by the invention has the advantages of rapidness, simpleness and convenience in operation, high enzymolysis efficiency and good separation effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the field of separating cuticles of plant leaves. Background technique [0002] The plant cuticle (cuticle) covers the stems, leaves, flowers and fruits of higher plants and is mainly composed of cutin and wax. The cuticle has the characteristics of acid and alkali resistance, oxidation resistance, impermeability to water and air permeability, so as the first protective barrier between plants and the external environment, it can resist external environmental factors, such as mechanical damage, ultraviolet rays and drought, and can also avoid external microbial infection. The cuticle can regulate the temperature of the plant body surface and the permeability of water, thereby regulating various physiological activities of the plant itself. The researchers found that the cuticle not only plays an important role in plant protection and development, but also that the flange features on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
CPCC12N5/04C12N2509/00
Inventor 林金星何丽娜吴鸿洋
Owner BEIJING FORESTRY UNIVERSITY
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