Ovarian cancer diagnosis or prognosis composition and application thereof

A technology of ovarian cancer and composition, applied in the field of biological diagnosis, to achieve the effect of increasing risk

Active Publication Date: 2019-08-13
王芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, there is no report on the combination of PDK1, PRKAA2 and PRKAB1 for the prognosis and diagnosis of ovarian cancer

Method used

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  • Ovarian cancer diagnosis or prognosis composition and application thereof
  • Ovarian cancer diagnosis or prognosis composition and application thereof
  • Ovarian cancer diagnosis or prognosis composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] High expression of PDK1, PRKAA2, PRKAB1 mRNA in ovarian cancer cell line (SK-OV-3) and ovarian cancer tissues

[0065] In order to explore the biological role of PDK1 in ovarian cancer cells, we detected the mRNA expressions of PDK1, PRKAA2, and PRKAB1 in the ovarian cancer cell line SK-OV-3 by real-time fluorescent quantitative PCR, and used the HFL cell line as a control.

[0066] In order to explore whether the expressions of ovarian cancer cells in vivo and ovarian cancer cells in vitro are consistent with PDK1, PRKAA2, and PRKAB1, we collected 17 ovarian cancer tissues and 15 ovarian benign tumor tissues, and analyzed the two groups of tissues by real-time fluorescent quantitative PCR. Perform mRNA detection.

[0067] (1) Reverse transcription reaction:

[0068] 20μl system: mix RNA (adjusted to 100μg-500μg) with 4μl 5×PrimeScript RT Master Mix, and make up to a total volume of 20μl with RNase-free water;

[0069] The reverse transcription conditions are 37°C for...

Embodiment 2

[0081] 2.1 Immunohistochemistry

[0082](1) Bake the specimen slices in an oven at 80°C for 40 minutes.

[0083] (2) The specimen slices were dewaxed twice in xylene, each time for 13 minutes; hydrated with gradient alcohol (100%, 100%, 90%, 80%, 70%), each time for 5 minutes, soaked in pure water twice, each time 5 minutes each time;

[0084] (3) High-pressure antigen retrieval: Take an appropriate amount of immunohistochemical antigen retrieval buffer (powder type citric acid method) (1500ml) that has been diluted into a working solution in a pressure cooker, place the pressure cooker on an induction cooker, and heat to boiling with high power. Adjust the power to medium (500-1000W), place the slices on the high temperature staining rack, put them into the boiling repair solution, cover the pot, buckle the pressure valve, and continue heating. Time 3 minutes when the pressure valve starts to blow air. Then take the pressure cooker away from the induction cooker, let it co...

Embodiment 3

[0150] Example 3 The relationship between the expression of PDK1, PRKAA2 and PRKAB1 and the survival of patients with ovarian cancer

[0151] 3.1 The relationship between the expression of PDK1 and the survival of patients with ovarian cancer

[0152] Group the patients according to the following grouping requirements, and count the survival time of the patients, the same below

[0153] The grouping requirements are as follows:

[0154] PDK1 low expression group: cases with (-) and (+) PDK1 expression detected by immunohistochemical method

[0155] PDK1 high expression group: cases with (++) and (+++) PDK1 expression detected by immunohistochemical method

[0156] The result is as Figure 8 , P=0.195, there was no significant difference in the survival time of ovarian cancer patients between the PDK1 low expression group and the PDK1 high expression group. It can be seen that the expression of PDK1 has no significant correlation with the survival and prognosis of ovarian c...

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Abstract

The invention discloses an ovarian cancer diagnosis or prognosis composition and application thereof. The ovarian cancer diagnosis or prognosis composition is composed of PDK1, PRKAA2 and PRKAB1. Theinvention provides the application of the ovarian cancer prognosis composition to preparation of an ovarian cancer diagnosis kit or a kit for predicating a prognosis condition of ovarian cancer. The kit for predicating the prognosis condition of the ovarian cancer comprises a reagent for detecting an expression amount of the PDK1, the PRKAA2 and the PRKAB1. The PDK1, the PRKAA2 and the PRKAB1 aredetected in a combined manner, and the prognosis of a patient with the ovarian cancer can be effectively judged; any index in single detection cannot be used for effectively judging the prognosis condition of the patient with the ovarian cancer. The composition disclosed by the invention provides effective prognosis indexes for clinical application.

Description

technical field [0001] The invention belongs to the field of biological diagnosis, and relates to a composition for diagnosing and prognosing ovarian cancer and its application. Background technique [0002] Ovarian cancer is the most lethal tumor among gynecological tumors, and its 5-year survival rate is less than 30%. [0003] The incidence of ovarian tumors ranks seventh among tumors that can be suffered by women, and ranks second among tumors of the female reproductive system, and its case fatality rate ranks fifth among all tumors that can be suffered by women. Ranked number one. In 2017, about 22,500 women in the United States were newly diagnosed with ovarian cancer, and 14,100 of them died of ovarian cancer. Ovarian cancer has become the leading cause of death from gynecological tumors in the United States. With the intensification of life and mental pressure given to women by society, coupled with economic and environmental impacts, the incidence and mortality of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158
Inventor 王芳姚莎莎尚文雯
Owner 王芳
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