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Preparation method of animal model with chronic atrophic gastritis

A technology of atrophic gastritis and animal models, applied in the field of medicine, can solve the problems of low success rate, long cycle, unsatisfactory model construction, etc., and achieve the effect of short construction cycle and stable pathological changes

Inactive Publication Date: 2018-12-18
右江民族医学院
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The main methods for constructing animal models of chronic atrophic gastritis include chemical carcinogenesis and Helicobacter pylori infection, etc., but so far, the construction of the model is not ideal, and there are still many areas worth exploring, such as the selection of experimental animals, the combination of drugs and The size of the dose, the length of modeling time, the ease of operation, etc.
N-methyl-N-nitro-nitrosoguanidine, ammonia water, sodium deoxycholate, sodium salicylate, etc. are currently commonly used chemical modeling materials, and Helicobacter pylori is the only pathogenic bacterium for constructing CAG animals. Both single and joint use can successfully build models, but the cycle is relatively long, and the model is not stable enough, and the success rate is relatively low

Method used

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  • Preparation method of animal model with chronic atrophic gastritis
  • Preparation method of animal model with chronic atrophic gastritis
  • Preparation method of animal model with chronic atrophic gastritis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Animal grouping: 220 6-week-old Kunming mice were randomly selected and divided into PBS control group, Helicobacter pylori group, N-methyl-N-nitro-nitrosoguanidine group, ammonia water group, and Helicobacter pylori group. group+base-N-nitro-nitrosoguanidine group+ammonia water group combined modeling group, male and female half and half.

[0016] 2. Immune intervention: The experimental group was fed 120g / mL N-methyl-N-nitroso-nitrosoguanidine at a concentration of 5mL / kg according to the body weight of the mice, once a day for 7 consecutive interventions, and 0.02wt. %ammonia. Control group: without any intervention, normal feeding.

[0017] 3. Bacterial infection: use clinically isolated Helicobacter pylori strains, which are identified as Helicobacter pylori by Gram staining, urease test, oxidase test, catalase test and 16S. The strains CagA and VagA are positive, and they are cultured The Helicobacter pylori was used for 3 days to prepare a concentration of 1...

Embodiment 2

[0026] Adopt the method of above-mentioned embodiment 1, Helicobacter pylori is poured 1 * 10 per mouse 8 CFU, N-methyl-N-nitro-nitrosoguanidine was administered to mice at a concentration of 100g / mL at a concentration of 5mL / kg, once a day, for 7 consecutive interventions, and 0.1wt.% ammonia water was used for feeding and drinking water. The experimental results are shown in Table 2.

[0027] Table 2 Modeling results of chronic atrophic gastritis

[0028]

Embodiment 3

[0030] Adopt the method of above-mentioned embodiment 1, Helicobacter pylori is poured 1 * 10 per mouse 9 CFU, N-methyl-N-nitro-nitrosoguanidine was administered to mice at a concentration of 100g / mL at a concentration of 5mL / kg, once a day, for 7 consecutive interventions, and 0.1wt.% ammonia water was used for feeding and drinking water. The experimental results are shown in Table 3.

[0031] Table 3 Modeling results of chronic atrophic gastritis

[0032]

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Abstract

The invention discloses a preparation method of an animal model with chronic atrophic gastritis. The preparation method comprises: filling mice with N-methyl-N-nitro-nitrosoguanidine, wherein ammoniawater is used as drinking water, carrying out proliferation on helicobacter pylori, wherein the concentration is adjusted through a BHI medium, perfusing each mouse in the experimental group with helicobacter pylori, wherein before intragastric administration, the mice fast and after intragastric administration, the mice are subjected to fasting and water deprivation, selecting the male and femalemice, carrying out pathological histological examination on gastric mucosa, separating and culturing helicobacter pylori from the gastric mucosa, and confirming helicobacter pylori colonization and inflammation formation. The preparation method has the advantages of short cycle, high success rate, stable pathological change, strong reproducibility and outstanding substantive features, and can provide a good animal model for studying diseases such as chronic atrophic gastritis and gastric cancer caused by helicobacter pylori infection.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a preparation method of an animal model of chronic atrophic gastritis. Background technique [0002] Chronic atrophic gastritis (CAG) is a precancerous disease of the stomach. Its onset is slow, lingering, protracted, and difficult to treat. If it is not well controlled, it may develop into intestinal metaplasia or atypical hyperplasia, and finally form stomach cancer. The research and treatment of CAG have attracted much attention. The modeling of CAG animal models is the key material for the basic and clinical research of the disease. A good animal model will provide direct in vivo evidence for the prevention and treatment of the disease. The main methods for constructing animal models of chronic atrophic gastritis include chemical carcinogenesis and Helicobacter pylori infection, etc., but so far, the construction of the model is not ideal, and there are still many areas w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K31/155A61K33/02A01K67/02
CPCA01K67/02A61K31/155A61K33/02A61K35/74A61K2300/00
Inventor 黄衍强黄干荣王露瑶梁凌玲赵丽娟周滢龙
Owner 右江民族医学院
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