Method for constructing yin-deficiency fire-excess type oral ulcer animal model based on sleep deprivation

A technology of yin deficiency and hyperactivity of fire, animal model, applied in the field of medical research, can solve the problems of short model duration, single evaluation index, etiology and clinical inconsistency, and achieve long-lasting effect

Inactive Publication Date: 2019-01-01
SUN YAT SEN UNIV
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Problems solved by technology

[0006] In order to overcome the above technical defects, the present invention provides a method for making animal models of oral ulcers caused by deficiency of yin and hyperactivity of fire due to lack of sleep. inconsistent defects

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  • Method for constructing yin-deficiency fire-excess type oral ulcer animal model based on sleep deprivation
  • Method for constructing yin-deficiency fire-excess type oral ulcer animal model based on sleep deprivation
  • Method for constructing yin-deficiency fire-excess type oral ulcer animal model based on sleep deprivation

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited thereto.

[0020] 1. Experimental materials

[0021] (1) Experimental animals: 50 SPF-grade adult male SD rats, weighing 200-260 g, were provided by Guangdong Medical Experimental Animal Center, license number: SCXK (Guangdong) 2013-0002.

[0022] (2) Experimental reagents: isoflurane, phenol, formic acid, chloral hydrate, normal saline, 5-hydroxytryptamine (5-HT)), γ-aminobutyric acid (GABA), corticosterone (CORT); adrenocorticotropic hormone ( ACTH), immunoglobulin M (IgM), malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) detection kits.

[0023] (3) Experimental equipment: self-made sleep deprivation device, anesthesia machine (MATRX), electronic analytical balance (Sartorius), infrared electronic thermometer (Corfu), refrigerator (Hair), plastic thin tube (length 6cm, inner...

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Abstract

The invention relates to a method for constructing a yin-deficiency fire-excess type oral ulcer animal model based on sleep deprivation. The method comprises the following steps: buccal mucosa of a rat is burnt by using phenol, so that white damage is caused, and an oral ulcer animal model is obtained after 24-48 hours; the animal model is placed in a multi-platform water-environment device for 2-7 days, wherein the multi-platform water-environment device is composed of a mouse box and a plurality of platforms, and the plurality of platforms are arranged in the mouse box and have diameters of4-8 cm and distance of 10-20 cm are arranged in the platform; and the place among the platforms is filled with water, and the height of the platform is about 0.1-2 cm higher than the water surface. The invention overcomes the defects that in the prior art, the evaluation index is single, the model duration time is short, the etiology is not matched with clinic, and the like.

Description

technical field [0001] The invention relates to a method for constructing an animal model in the field of medical research. Background technique [0002] Chinese medicine believes that the substances and functions that have the functions of nourishing, coagulating, and suppressing yang heat on the human body belong to yin. Yin-deficiency syndrome refers to the pathological state in which the body's yin-fluid is depleted and yin does not control yang, resulting in relative hyperactivity of yang, dryness, heat, elevation, movement, and hyperactivity of function deficiency. "Compendium of Materia Medica" says "seven injuries, food injury, sadness, drinking injury, atrioventricular injury, hunger injury, fatigue injury, meridian, camp and Wei Qi injury are the seven injuries, and the seven injuries all affect the true yin." At present, the rise of virtual fire caused by staying up late in human beings is common. Modern medical research on the pathophysiology of yin deficiency ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61D1/00A61K31/05
CPCA01K67/02A61D1/00A61K31/05A01K67/027A01K2207/20A01K2227/105A01K2267/0368A01K67/00A01K2207/35A01K2267/03
Inventor 姚宏亮谌攀李沛波苏薇薇
Owner SUN YAT SEN UNIV
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